刺五加注射液对小鼠全脑缺血再灌注损伤的影响

目的: 探讨刺五加注射液对脑缺血再灌注损伤的影响。方法: 采用小白鼠双侧颈总动脉及颈部软组织结扎术复制全脑缺血再灌注损伤的动物模型。动物随机分为3组:对照组、缺血再灌注组及刺五加组,在实验过程中动态观测小白鼠脑电图,测量大脑皮层脑含水量和脑组织伊文思蓝含量。结果: 对照组小白鼠脑电图在手术前后无任何异常变化,缺血再灌注组和刺五加组小白鼠脑电图观察过程中均呈直线;缺血再灌注组脑含水量(83.92±5.10)%高于刺五加组(79.21±2.59)%(P<0.05);缺血再灌注组伊文思蓝含量(0.535 7±0.098 8)明显高于刺五加组(0.300 0±0.064 5)(P<0.01)。结论: 刺五加注射液对小白鼠全脑缺血再灌注损伤具有保护作用。

Influence of acanthopanax senticosus injection for cerebral ischemic reperfusion injury in mice

Objective: To study the influence of acanthopanax senticosus injection (ASI) on cerebral ischemic reperfusion injury in mice.Methods: The cerebral ischemic reperfusion injury model was established by binding the bilateral total artery and the soft tissue of neck in mice.The mice were randomly divided into 3 groups:control group,ischemic reperfusion group (I/R) and ASI group(AS).The electroencephalogram(EEG) was continually observed during the experiment course and the water content in cerebrum and the Evans blue content in brain organization were measured after the mice were killed.Results: The mice EEG in control group did not have any unusual change before and after operation;the mice EEG in I/R group and AS group was a straight line during the observing course;the water content in I/R group(83.92±5.10)% was higher than that in AS group(79.21±2.59)% (P<0.05);the Evans blue content in I/R group(0.535 7 ±0.098 8) was obvious higher than that in AS group(0.300 0 ±0.064 5) (P<0.01).Conclusions: The ASI plays a protective role in ischemic reperfusion injury in mice.