磷酸钙钠/β-磷酸三钙陶瓷支架接种骨髓基质细胞成骨性能的研究

目的：观察磷酸钙钠／β-磷酸三钙(NaCaPO2/β-TCP)支架接种骨髓基质细胞后植入裸鼠皮下的成骨性能。方法：通过理化方法,将煅烧牛松质骨转化为NaCaPO2/β-TCP双相钙磷陶瓷。获取兔髂骨松质骨骨髓基质细胞,体外分离、扩增、诱导后,接种于NaCaPO2/β-TCP支架。将支架／细胞复合物植入裸鼠背部皮下,NaCaPO2/β-TCP陶瓷单纯植入作为对照。植入后4、8周取材,通过大体、组织学观察,评价成骨活性。结果：支架／细胞复合物植入后4周,材料表面见相对较成熟的骨组织,内部主要为软骨;植入后8周,大量骨小梁形成。可见骨髓腔、骨髓细胞及脂肪细胞,在支架材料和骨组织的邻接区域见成骨细胞及破骨细胞。可见软骨内成骨方式。结论：NaCaPO2/β-TCP支架接种骨髓基质细胞后显示良好的成骨活性,能够促进未成熟骨矿化,可以作为骨组织工程支架材料。

Bone formation by seeding bone marrow stromal cells on the sodium calcium phosphate/beta tricalcium phosphate scaffold

PURPOSE: To observe the osteogenesis of sodium calcium phosphate/beta-tricalcium phosphate scaffold loaded with marrow stromal cells in a nude mice subcutaneous model. METHODS: To transform the sintered bovine cancellous bone into NaCaPO(4)/beta-TCP biphasic calcium phosphate ceramic by physical and chemical methods. Bone marrow stromal cells (BMSCs) were obtained by harvesting the cancellous bone from the iliac crest of a rabbit. Then the BMSCs were isolated, expanded and induced in vitro and seeded in the NaCaPO(4)/beta-TCP ceramic scaffolds. The scaffold/BMSCs composites were implanted subcutaneously into the backs of nude mice. Implantation of NaCaPO(4)/beta-TCP ceramic alone was acted as control. At 4 and 8 weeks after implantation, the specimens were harvested and the osteogenetic activity were evaluated by gross and histologic observation. RESULTS: At 4 weeks after implantation, the relatively mature bone were seen on the surface of the material, the inner part of the material were mainly cartilaginous bone. At 8 weeks after implantation, A large amount of trabecula bone had formed and the medullary cavity, medullary cells and lipocytes could be seen. Osteoblasts and osteoclasts were seen between the scaffolds and the local bone in the transition area. The endochondral bone formation pattern were observed. CONCLUSIONS: The NaCaPO(4)/beta-TCP scaffold/BMSCs composites showed good osteogenetic activity and could promote mineralization of the immature bone. It can be used as the bone tissue engineering scaffolds.