环磷酰胺对大鼠骨密度和骨微结构的影响

目的：研究不同剂量环磷酰胺对雌性SD大鼠骨密度和骨微结构的影响。方法40只3月龄SD雌性大鼠随机数字表法分为环磷酰胺低剂量组、中剂量组、高剂量组和对照组，每组各10只，环磷酰胺各组分别腹腔注射不同剂量（5 mg／kg、8 mg／kg、10 mg／kg）的环磷酰胺，对照组腹腔注射等量生理盐水，每天一次，连续15天，观察大鼠骨密度及骨微结构等指标。结果不同剂量的环磷酰胺组与对照组比较，全身骨密度分别下降了9．8％、13．3％和15．6％；股骨骨小梁数目分别下降了49．8％、65．5％和72．8％；股骨骨小梁分离度则分别上升了225．2％、416．9％和577．4％。结论环磷酰胺可使大鼠骨密度降低，骨小梁数量减少，骨微结构受损，且环磷酰胺剂量越大，骨微结构受损越严重。

Effect of cyclophosphamide on bone mineral density and bone microstructure in rats

Objective To investigate the effect of different doses of cyclophosphamide ( CP) on bone mineral density ( BMD) and bone microstructure in female SD rats.Methods Forty SD 3-month-old female rats were randomly divided into low dose of CP group, middle dose of CP group, high dose of CP group, and control group ( n=10 in each group) .Rats in the CP groups were intrapentonally injected with different doses of CP (5, 8, or 10 mg/kg, respectively).Rats in control group were intrapenitoneally injected with the same volume of normal saline.The injection was once a day, and lasted for 15 days.BMD and bone microstructure in rats were observed.Results BMD of the total body in different CP dose groups declined by 9.8%, 13.3%, and 15.6%, respectively, compared to that in control group.Trabecular number in the femur declined by 49.8%, 65.5%, and 72.8%, respectively.Trabecular separation of the femur increased by 225.2%, 416.9%, and 577.4%, respectively.Conclusion CP may decrease BMD and trabecular number in rats, and demage bone microstructure.The higher dose of cyclophosphamide, the more serious the damage of bone microstructure.