贵州水族人群D13S317、D7S820和D16S519 STR Loci遗传多态性

目的：调查贵州水族人群D13S317、D7S820和D16S519 STR基因座遗传多态性。方法：采用Chelex-100快速提取法提取贵州省荔波县水尧水族乡44名水族无关个体EDTA抗凝血标本DNA，再通过PCR复合扩增，变性聚丙烯酰胺凝胶电泳和银染分型，最后用Modified—Powerstats统计软件包输出各群体遗传学数据。结果：贵州水族人群D13S317，D7S820和D16S519基因座分别检出7个、6个和6个等位基因，各基因座基因型分布均符合Hardy—Weinberg平衡定律（P〉0．05）。D13S317，D7S820和D16S519基因座的杂合度分别为0．636，0．818和0．818；个体识别律分别为0．909，0．893和0．876；多态信息量分别为0．74，O．75和0．75；非父排除率分别为：0．337，0．633和0．633。结论：贵州水族D7S820和D16S519两个基因座有较高的遗传多态性，在法医学的个体识别和群体遗传学的研究中有较高的应用价值。

Genetic Polymorphism on STR Loci (D13S317, D7S820 and D16S519) of Shui Population in Guizhou Province

Objective： To investigate the genetic polymorphism on STR Loci （ D13S317, D7S820 and D16S519） of Shui population in Guizhou Province. Methods： Using the Chelex - 100 fast extraction, we extracted EDTA anticoagulant blood specimen DNA from 44 unrelated individuals of Shui nationality in Shuiyao of Libo county in Guizhou province; and then amplified by the PCR; and typed with denaturing polyacrylamide gel electrophoresis and silverstaining; finally output the genetics data of each population with Modified - Powerstats software. Results： STR loci （ D13 S317, D7 S820 and D16S519 ） of Shui population in Guizhou province were detected 7, 6 and 6 alleles, whose genotype distribution rule conformed to the Hardy - Weinberg balance rule （P 〉 0. 05） . And the heterozygosity （H） of D13S317, D7S820 and D16S519 are 0. 636, 0. 818 and 0. 818; the discrimination power （DP） are 0. 909, 0. 893 and 0. 876; polymorphism information content （PIC） are 0. 74, 0. 75 and 0. 75 ; and probabilities of paternity exclusion （PPE） are 0. 337, 0. 633 and 0. 633. Conclusion： There are greater genetic polymorphism on D7S820 and D16S519, which is valuable for individual discrimination of forensic medicine and the study of population genetics.