大鼠口服裸花紫珠后木犀草素的体内药动学研究

目的:建立木犀草素血药浓度的高效液相色谱测定方法,并探讨大鼠口服裸花紫珠提取物后木犀草素的体内药代动力学行为。方法:采用phenomenex C18(5u,250mm×4.6mm)色谱柱,流动相为甲醇-水(52∶48),流速1.0 mL·min-1,柱温:30℃,检测波长350nm。大鼠灌胃裸花紫珠提取物20g·kg-1后,与不同时间点股静脉取血,用HPLC法测定其血药浓度,绘制药-时曲线。结果:大鼠血浆中,木犀草素在0.005～0.10μg范围内,回归方程为Y=29063X-1933.3,r=0.9989,呈良好的线性关系。大鼠灌胃裸花紫珠提取物后,木犀草素的药-时曲线符合二室模型。结论:裸花紫珠提取物中木犀草素在大鼠体内吸收迅速。该方法专属性强,灵敏度高,可用于木犀草素在大鼠体内的药动学研究。

Pharmacokinetic study of luteolin from Callicarpanudiflora in rats

Objective:To establish an HPLC method for the determination of luteolin in rat plasma and to study the pharmacokinetics of luteolin in rats.Methods:An phenomenex C18(5u,250 mm×4.6 mm) column was adopted,the mobilephase was a mixture of methyl alcohol and water(52∶48) at the flow rate of 1.0 mL/min and the UV detector was operated at 350 nm.After oral administrat-ion of Callicarpanudiflora(20 g/kg),the luteolin concentration in rat plasma was detected by HPLC.The pharmacokinetic parameters were calculated by WinNonlon 5.2 program.Results:In rat plasma,a good linearity of luteolin was found in the range of 0.005-0.10 μg(r=0.9989).The plasma luteolinconcentration-time curve could be evaluated by the two compartment modes.Conclusions:Luteolin can be absorbed quickly in rats.It is specific and sensitive,and can be used in the pharmacokinetic study of luteolin of rat in vivo.