Twist、E—cadherin及Vimentin在表达及意义宫颈鳞状上皮癌变的

目的探讨宫颈鳞状上皮癌变过程中Twist、E-cadherin、Vimentin的表达规律及临床意义。方法以慢性宫颈炎为对照，检测宫颈上皮内瘤变（CIN）及宫颈鳞状细胞癌组织标本中Twist（原位杂交）、E-cadherin、Vimentin（SABC免疫组化法）的表达情况，分析其相关性。结果（1）慢性宫颈炎、CINI、CINII、CINIU及宫颈癌组中：Twist的阳性率分别为10．O％、28．1％、29．6％、42．9％、64．3％，其中Twist在宫颈鳞癌的表达均明显高于其他组（P〈0．05）。E-cadherin、Vimentin的阳性率分别为80．O％、68．8％、55．6％、51．4％、39．3％和0、6．3％、7．4％、31．4％、32．1％，其中两者在宫颈鳞癌组、CINⅢ组中的表达与慢性宫颈炎组比较差异均有统计学意义（P〈0．05），（2）分析全部CIN及宫颈癌病例，Twist的表达与E-cadherin呈负相关（r=-0．37，P〈0．01），与Vimentin呈正相关（r：0．23，P〈0．05）。结论宫颈组织中Twist的异常表达与宫颈鳞状上皮癌变发生关系密切，Twist可能通过调控E-cadherin、Vimentin的表达参与宫颈鳞状细胞癌的发生。

Expression and significance of Twist and E - cadherin and Vimentin in cervical squamous epithelial cancerization H

Objective To explore the expression rule and clinic significance of Twist and E-cad- herin and Vimentin in cervical squamous epithelial cancerization. Methods Chronic cervicitis were used as control group, the expression of Twist （using hybridization in situ）, E-cadherin and Vimentin （SABC im- munohistochemical stain） in tissues of cervical intraepithelial neoplasia （CIN） and cervical squamous carci- noma were detected, and the correlations were analyzed. Results The positive rate of Twist in group of chronic cervicitis, CIN Ⅰ , CIN Ⅱ, CINⅢ and cervical squamous carcinoma was 10. 0% ,28.1% ,29. 6%, 42. 9% and 64. 3%, respectively. The expression rate of Twist in cervical squamous carcinoma group was higher than other groups （ P 〈 0. 05 ）. The positive rates of E-cadherin and Vimentin were 80. 0%, 68. 8%, 55.6%, 51.4%, 39. 3% and 0,6. 3% ,7.4% ,31.4% ,32. 1%, respectively. The difference of E-cadherin and Vimentin expression between the cervical squamous carcinoma （or CIN III group） and the chronic eervicitis group was obvious （ P 〈 0.05, respectively）. The expression of Twist was negatively cor- related with E-cadherin（ r = -0. 37, P 〈0. 01 ）, and it was positively correlated with Vimentin in all ca- ses of CIN and cervical squamous carcinoma（ r = 0. 23, P 〈 0. 05）. Conclusions There is a close rela- tionship between abnormal expression of Twist and cervical squamous epithelial cancerization. Twist may participate in the genesis of cervical squamous carcinoma through mediating the expression of E-cadherin and Vimentin in cervix tissue.