尿毒症患者血清对内皮细胞凋亡的影响及rhEPO的干预作用

目的 探讨尿毒症患者血清对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)凋亡的影响及重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)的干预作用.方法 选择我院2008年12月至2009年4月收治的尿毒症患者和健康志愿者各10例,HUVECs按不同的干预方式分3组进行实验:对照组(含10%健康者血清)、尿毒症组(含10%尿毒症患者血清)、rhEPO干预组(rhEPO 5、10、15 U/ml,分别加入含有10%尿毒症患者血清的培养基).各组干预24 h,TUNEL法检测细胞凋亡,比色法测定细胞内活性氧.结果 尿毒症患者血清环境中内皮细胞的凋亡指数和活性氧水平均高于对照组(P<0.01).rhEPO可抑制尿毒症患者血清诱导的内皮细胞凋亡(P<0.01),降低细胞内活性氧水平(P<0.01);随着rhEPO浓度的增加,内皮细胞凋亡指数和ROS水平都逐渐降低(P<0.05).相关分析显示:凋亡指数与活性氧水平呈正相关(r=0.89,P<0.01).结论 尿毒症患者血清可以诱导HUVECs凋亡,其机制可能与氧化应激有关.rhEPO可能通过减轻氧化应激反应而抑制尿毒症患者血清诱导的内皮细胞凋亡.

Effect of uremic serum on endothelial cell apoptosis and intervention of recombinant human erythropoietin in the process

Objective To explore the effect of uremic serum on apoptosis in human umbilical vein endothelial cells(HUVECs) and the intervention by recombinant human erythropoietin(rhEPO) in this process.Methods From Dec.2008 to Apr.2009,10 uremic patients and 10 healthy volunteers were enrolled in this study.HUVECs were divided into 3 groups:control group(including 10% healthy serum medium),uremic group(including 10% uremic serum medium) and rhEPO treatment group(rhEPO at 5,10 or 15 U/ml was added to 10% uremic serum m...