蛋白质组学研究技术及其联合应用

蛋白质组学是研究细胞、组织和器官内所有蛋白质的组成及其动态变化的科学,是在蛋白质水平上定量的、动态的、整体的研究生物体。目前蛋白质组学技术分为样品制备、分离和鉴定3个方面,其新技术主要有激光捕捉显微解剖法、离心超滤法、双向凝胶电泳、同位素亲和标签技术、色谱技术以及质谱技术等。然而,任何一种蛋白质组学研究技术都有其缺陷。因此多种技术的联合应用能使蛋白质组研究更精确和完整,是蛋白质组学的发展趋势。

Advances in Research of New Proteomics Technologies and Their Combined Application

Proteomics is the study of constitution of all proteins and their dynamic changes in cells,tissues or organs.It quantitatively and dynamically studies an organism as a whole at the protein level.The proteomics technologies include the preparation,separation and identification of samples.The new technologies involve laser capture microdissection,centrifugal ultrafiltration,two-dimensional gel electrophoresis,isotope-coded affinity tag,chromatography,mass spectrometry.However,each of the proteomics technologies has its limitations.Therefore the combined use of two or more technologies can achieve better precision and completeness and it represents the trend of the proteomics study.