The influence of properties of packing materials upon the recovery of biological substances isolated from urine by solid-phase extraction |
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| Authors: | Bogus&#x;aw Buszewski |
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| Institution: | Department of Chemical Physics, Faculty of Chemistry Maria Curie Sklodowska University, Pl-20 031 Lublin, Poland |
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| Abstract: | A series of packing materials with alkyl phase chemically bonded to silica gels of various porosity have been prepared. These packings have been used to isolate the test substances 5-hydroxyindole-3-acetic acid (5-HIAA) and serotonin (5-HT) from urine. The influence of the support porosity, structure of chemically bonded phase, length of alkyl chain, and coverage density of the recovery of isolated substances was studied. |
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| Keywords: | Chemically bonded phase liquid chromatography solid-phase extraction hydroxyindoles isolation from urine |
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