睾丸生精细胞完全体外成熟培养的研究

目的在动物实验研究的基础上对人类睾丸生精细胞在体外培养分化、成熟进行初步探索,为临床治疗无精子症奠定基础.方法将14例患者的睾丸组织分离成生精细胞混悬液,采用生精细胞与支持细胞共培养或分选出初级精母细胞和精子细胞培养,观察培养前后各级生精细胞的分化或成熟情况.结果14例患者睾丸生精细胞经体外培养后均有不同程度的分化和成熟.初级精母细胞能分化为精子细胞,分化率平均为4.54%～6.51%;Sa期精子细胞能变形、成熟为Sc期精子细胞,成熟率为3.13%～5.74%.结论人类生精细胞经体外培养能进一步分化、成熟.初级精母细胞能分化为早期精子细胞,Sa期精子细胞可变形为更成熟形式的Sc期精子细胞.甚至可以通过使用被拉长的精细胞或圆形细胞进行显微受精,以达到妊娠的目的.

Differentiation and maturation of testicle spermatogonia in vitro

Objective To carry out in vitro culture and in vitro maturation of human spermatogonla tor treating azoospermia infertility in clinic. Methods The testicle tissue from 14 patients was dissociated into spermatogonia cells mixture, co-cultured with sertoli cells, or the primary spermatocytes and sperm cells were selected out to co-culture. The maturation or differentiation of spermatogenic cells at different stages was observed before and after in vitro culture. Results There was differentiation and maturation of spermatogenic cells in many degrees after in vitro culture. Primary spermatocytes could grow into sperm cells, with differentiation rate of 4.54% to 6.51% ; The sperm cells at Sa stage could growth into sperm cells, with maturation rate of 3.13% to 5.74%. Conclusion Human spermatogonia can differentiate and maturate during culture in vitro. Primary spermatocytes can grow into nonage sperm cells, and sperm cells at Sa stage can metamorphose and mature into sperm cells at Sc stage.