| RP-HPLC法测定7种中药材中反式阿魏酸和顺式阿魏酸的含量 |
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| 引用本文： | 刘晓芳,刘养清,赵平,马春红,陈元元,仝永斌.RP-HPLC法测定7种中药材中反式阿魏酸和顺式阿魏酸的含量[J].现代中药研究与实践,2009,23(2). |
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| 作者姓名： | 刘晓芳 刘养清 赵平 马春红 陈元元 仝永斌 |
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| 作者单位： | |
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| 基金项目： | 山西省卫生厅科技攻关项目 |
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| 摘 要： | 目的建立7种中药材中反式阿魏酸和顺式阿魏酸含量测定方法并进行比较。方法C18色谱柱(4.6mm×250mm,5μm),以乙腈-0.3%醋酸(20∶80)为流动相,检测波长为320nm。结果阿魏酸的线性范围为0.0285～0.513μg(r=0.9999),平均回收率为98.17%,RSD为0.73%(n=6)。结论该方法操作简便准确,重复性好,可以快速测定中药材中阿魏酸的含量。
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| 关 键 词： | 反式阿魏酸 顺式阿魏酸 高效液相色谱法 |
Determination of Trans-ferulic Acid and Cis-ferulic Acid in 7 Medicinal Materials by RP-HPLC |
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| LIU Xiao-fang,LIU Yang-qing,ZHAO Ping,MA Chun-hong,CHEN Yuan-yuan,TONG Yong-bin.Determination of Trans-ferulic Acid and Cis-ferulic Acid in 7 Medicinal Materials by RP-HPLC[J].Research and Practice on Chinese Medicines,2009,23(2). |
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| Authors: | LIU Xiao-fang LIU Yang-qing ZHAO Ping MA Chun-hong CHEN Yuan-yuan TONG Yong-bin |
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| Abstract: | Objective To develop HPLC method for determination of trans-ferulic acid and cis-ferulic acid in 7 medicinal materials .Methods Analysis was carried out on a C18 (4.6mm×250mm,5μm)column eluled with acetonitrile-0.3 % acetic acid (20:80) as the mobile phase , the detection wavelength was set at 320 nm. Results The linear relationship of ferulic acid was in the range of 0.028 5～0.513 μg ( r = 0.999 9) ,the recovery was 98.17% ,RSD was 0.73%( n = 6). Conclusion The method is simple ,accurate and reproducible, and can be used for the analysis of ferulic acid in medicinal materials |
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| Keywords: | trans-ferulic acid cis-ferulic acid HPLC |
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