伯氏疟原虫抗体芴醇株抗性转化相关基因的克隆

目的：研究药物敏感虫株伯氏疟原虫K173株及其在本芴醇压力下的本芴醇抗性株之间基因表达的差异状况，克隆抗性相关基因，方法：采用mRNA差异显示技术寻相关基因，并用Northern杂交证实，结果：采用24种引物组合进行mRNA差异显示，克隆到30余个差异表达基因片段，对其中8个进行了Northern杂交鉴定及测序，结果表明，与敏感株相比，克隆CB52在本芴醇抗性株中表达明显增多，而在氯喹抗 株中的表达有所减少，说明克隆CB52确与本芴醇抗性相条新基因序列，结论：克隆CB52基因表达水平的改变与伯氏疟原虫本芴醇抗性的产生相关，疟原虫原本芴醇的抗性机制在基因水平同于氯喹抗性。

Cloning of genes of Plasmodium berghei associated with benflumetol resistance

Objective:To isolate genes related to benflumetol resistance in the rodent malaria P.berghei K173 strain.Methods:The mRNA differential display technique was used to identify differentially expressed genes between drug sensitive P.berghei K173 strain and benflumetol resistant strain selected under benflumetol pressure.Northern blot was performed in order to determine the genes actually derived from maternal strains.Results:PCR reactions were prepared respectively with twenty four pairs of primers in each of the strains.Thirty three differentially displayed genes fragments were cloned and eight of them were sequenced. The eight sequences were novel ESTs and were submitted to GenBank. Homology searching revealed that clone CB52 was slightly homologous to P.falciparum cyclophilin gene. Hybridization confirmed that the gene fragment CB52 was upregulated in the benflumetol resistant parasite strain, while downregulated in the chloroquine resistant strain.Conclusions:CB52 fragment was involved in the benflumetol resistance in P.berghei and the genetic mechanisms of drug resistance to benflumetol and chloroquine were different.