| 亲水作用色谱-蒸发光散射法测定益母草中盐酸水苏碱的含量 |
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| 引用本文： | 陈碧莲,祝明,黄卫国,郭增喜. 亲水作用色谱-蒸发光散射法测定益母草中盐酸水苏碱的含量[J]. 药物分析杂志, 2012, 0(3): 502-504 |
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| 作者姓名： | 陈碧莲 祝明 黄卫国 郭增喜 |
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| 作者单位： | 浙江省食品药品检验所;浙江中医药大学 |
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| 基金项目： | 国家“十一五”重大新药创制专项(课题编号:2009ZX09308-001) |
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| 摘 要： | 目的:建立亲水作用色谱-蒸发光散射检测器联用测定益母草中盐酸水苏碱含量的方法。方法:采用HILIC柱(250mm×4.6 mm,5μm);乙腈-0.2%冰醋酸溶液(80∶20)为流动相;Alltech2000蒸发光散射检测器:漂移管温度80℃,气体流速2.0 L.min-1;流速0.5 mL.min-1。结果:盐酸水苏碱进样量在0.536～10.713μg范围内呈良好的线性关系,平均回收率为101.9%,RSD为1.4%。结论:此方法简便、灵敏度高,重现性好,可用于益母草的含量测定。
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| 关 键 词： | 亲水作用色谱(HILIC) 益母草 盐酸水苏碱 强极性化合物分析 标准改进 |
HILIC - ELSD determination of Stachydrine hydrochloride in Herba Leonuri |
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| CHEN Bi-lian,ZHU Ming,HUANG Wei-guo,GUO Zeng-xi. HILIC - ELSD determination of Stachydrine hydrochloride in Herba Leonuri[J]. Chinese Journal of Pharmaceutical Analysis, 2012, 0(3): 502-504 |
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| Authors: | CHEN Bi-lian ZHU Ming HUANG Wei-guo GUO Zeng-xi |
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| Affiliation: | 1(1.Zhejiang Institute for Food and Drug Control,Hangzhou 310004,China; 2.Zhejiang University of Traditional Chinese Medicine,Hangzhou 310053,China) |
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| Abstract: | Objective: To establish a HILIC-ELSD method for determination of Stachydrine hydrochloride in Herba Leonuri.Method: The separation was performed on a HILIC column(250 mm ×4.6 mm,5 μm)with acetonitrile-0.2% glacial acetic acid solution(80∶ 20)as mobile phase at a flow rate of 0.5 mL·min-1.An Alltech 2000 evaporative light-scattering detector(ESLD)was used as detector with gas flow rate of 2.0 L·min-1 and drift tube temperature of 80℃.Results: There was a good linear relationship with the range of 0.536–10.713 μg.The average recovery was 101.9%(RSD = 1.4%).Conclusion: The method is simple,sensitive and reliable,it can be used as quantitative analysis method for Herba Leonuri. |
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| Keywords: | HILIC stachydrine hydrochloride Herba Leonuri strongly polar compound assay standard improvement |
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