髓样细胞表达触发受体1在重症急性胰腺炎中的表达和意义

目的探讨髓样细胞表达触发受体1(TREM-1)在重症急性胰腺炎(SAP)中的表达和意义。方法反转录聚合酶链反应(RT-PCR)检测轻型胰腺炎、重症急性胰腺炎患者和正常人外周血TREM-1 mRNA的表达,酶联免疫吸附实验(ELISA)检测SAP外周血中可溶性TREM-1、α和TNF-IL-10的含量。结果 SAP患者外周血中TREM-1 mRNA的相对表达显著高于轻型胰腺炎和正常人对照组(P0.05);患者外周血中TREM-1与TNF-α和IL-10的含量均呈正相关,相关系数分别是0.951SAP(P0.05)和0.873(P0.05)。结论 TREM-1在SAP患者外周血中的呈高表达,与TNF-α和IL-10关系密切,具有重要的临床意义。

Significance and expression of triggering receptor expression on myeloid cells-1 in severe acute pancreatitis

Objective To investigate the significance and expression of triggering receptor expression on myeloid cells-1（TREM-1）in severe acute pancreatitis（SAP）.Methods RT-PCR analysis was used to test the expression of TREM-1 mRNA in peripheral blood of mild acute pancreatitis,SAP and the normal control,and ELISA assay was applied to detect the dose of TREM-1,TNF-αand IL-10 in peripheral blood of SAP patients.Results The expression level of TREM-1 mRNA in SAP group was higher dramatically than that of mild acute pancreatitis group（MAP）and the control group.The dose of TREM-1,TNF-α and IL-10 in SAP patients presented the positive correlation with the correlation coefficient being respective 0.951（P0.05）and0.873（P0.05）.Conclusion The expression level of TREM-1 mRNA is higher in SAP patients,and its dose has positive corre-lation with TNF-α and IL-10,which suggests the important clinical significance for SAP patients.