代谢型谷氨酸受体5mRNA与脑梗死的相关性研究

目的：观察急性脑缺血大鼠脑组织中mGluR5 mRNA在不同时段的表达变化的规律，探讨mGluR5 mRNA与脑梗死的相关性。方法：75只雄性Wistar大鼠按照随机数字表法分为正常对照组、手术组1、3、6、12、24 h及3 d、14 d组及其相对应的假手术对照组，采用大鼠MCAO模型，利用原位杂交技术，检测各组mGluR5 mRNA的表达。结果：与正常对照组相比，假手术对照组mGluR5 mRNA表达无明显改变（P〉0.05）。手术组mGluR5 mRNA各时间段表达均显著高于假手术对照组及正常对照组，缺血1 h其表达即有升高，比较差异均有统计学意义（P〈0.01）。mGluR5 mRNA表达6 h达到高峰（F=5.328，P〈0.00），3 d后表达开始下降（P〈0.01），14 d基本降至正常对照水平（P〉0.05）。结论：mGluR5在脑缺血后升高，提示mGluR5在脑梗死中有着重要作用，从而对我们的临床研究指出一条途径。

The Study on Relationship between Metabotropic Glutamate Receptor 5 mRNA and Cerebral Infarction

Objective：To investigate the correlation between mGluR 5mRNA and cerebral infarction.Method：Seventy-five male Wistar rats were randomly divided into normal control group,operation group 1,3,6,12,24 h and 3 d, 14 d groups and corresponding sham operation control group,the rat MCAO model was used in situ hybridization,was used to detect the expression of mGluR5 mRNA.Result：The normal group and the sham operation group had no difference at each time（P〉0.05）. Each time the operation group mGluR5 mRNA expression were higher than the sham operation control group and normal control group,ischemia 1 h and its expression is increased,and the difference were all statistically significant （P〈0.05）. The mGluR5 mRNA expression reached peak at 6 h（F=5.328,P〈0.00）,began to decline after 3 d（P〈0.01）, the basic dropped to normal control level after 14 d（P〉0.05）.Conclusion：MGluR5 increased after cerebral ischemia, suggesting that mGluR5 plays an important role in cerebral infarction,so as to point out a way for our clinical study.