糖皮质激素联合白芍总苷对大鼠系膜增生性肾炎的治疗作用

目的观察糖皮质激素联合白芍总苷(TGP)对大鼠系膜增生性肾小球肾炎(MsPGN)的治疗作用。方法采用胃肠道综合免疫方法建立大鼠MsPGN模型,以强的松为对照,观察激素和TGP联合应用对大鼠蛋白尿、血生化指标以及肾组织形态学改变的影响。蛋白尿采用双缩脲法测定,血生化指标由半自动生化分析仪测定,肾组织形态学为普通光镜观察。结果模型组大鼠出现大量蛋白尿,血肌酐(Cre)、尿素氮(Bun)、转氨酶(ALT)和甘油三酯(TG)升高,血浆总蛋白(TP)和白蛋白(Alb)下降,模型组的肾小球系膜细胞中至重度增生,系膜基质聚积。灌胃给予强的松和TGP(1mg/kg 100mg/kg)联合治疗4周后,肾小球系膜细胞增生、基质聚积明显减少,大鼠的蛋白尿减轻,Cre、Bun、ALT和TG下降,TP和Alb上升,与单独使用TGP(100mg/kg)、强的松(5mg/kg)有类似作用。结论TGP与强的松联用,可降低强的松的用量,对于MsPGN大鼠的肝肾功能有明显改善作用,并能部分逆转受损肾小球的病理改变。

The effect of total glucosides of paeony and glucocorticoid on the mice with mesangial proliferative glomerulonephritis

Obiective To observe the effect of glucocorticoid and total glucosides of paeony(TGP)on the renal in-jury in mesangial proliferative glomerulonephritis(MsGPN) rats,and provide experiment evidence for the treatment of MsPGN with glucocorticoid combined TGP.Methods The MsPGN model was established by gastrointestinal comprehensive immunization,glucocorticoid was given in control group, Urinary protein, blood biochemical parameters and the pathology changes of renal were observed.Results Compared with normal group,the urine protein, The blood createnine(Cre) urea nitrogen(Bun),alanine amineotransferase(Alt)and triglyceride(TG) increased,while theserum total protein(TP)and albumin(Alb)decreased in model group. The mesangial cells(MC)was found with moderate to severe proliferation and extracellular matrix(ECM) accumulated in glomeruli. After treatment with glucocorticoid combined TGP(1 mg/kg+100 mg/kg)for four weeks, the damage of the renal structure alleviated markedly in treatment group, its urine protein,Cre,Bun,Tg and Alp decreased while its TP and Alb increased.There is similar effect of the therapy between the glucocorticoid combined TGP group and the group treated with TGP(100 mg/kg) or glucocorticoid(5 mg/kg) only.Conclusion glucocorticoid and TGP can degrade the dose of glucocorticoid, improve the Hepatic and renal function and partly revert the glomeruluar histopathological changes in rats with MsPGN.