卵巢上皮性癌患者外周血中癌细胞的检测

目的 检测卵巢上皮性癌患者外周血干细胞（ＰＢＳＣ）中有无癌细胞污染及原发晚期卵巢上皮性细胞患者外周循环血中有无癌细胞。方法 应用卵白素－生物素酶－复合物（ＡＢＣ）法,检测２例患者ＰＢＳＣ及８例患者外周血。为了确定ＡＢＣ法检测血中癌细胞的灵敏度,分别用卵巢癌细胞系ＳＫＯＶ３,３ＡＯ以及从浆性乳头状腺癌,未分化腺癌虱腹水中得到的原代癌细胞,制成癌细胞与单核细胞的不同浓度模型,应用单克隆抗体ＣＯＣ１８３

Detection of tumor cells in peripheral blood for patients with ovarian epithelial carcinoma

Objective To detect tumor cell contamination of peripheral blood stem cell (PBSC) and the presence of tumor cells in peripheral circulating blood in patients with primary advanced ovarian cancer. MethodsTo establish limits of sensitivity of the immunocytochemical stainings technique(ABC method) for tumor cell detection, four types of ovarian cancer tumor cell(ovarian cancer cell lines SKOV3, 3AO, primary cancer cell from ascites of serous papillary carcinoma and undifferentiated adenocarcinoma) were added into normal blood to prepare the models with different ratios of tumor cells to mononuclear cells. Monoclonal antibodies(MAB) of COC 183 B2, CK(AE1/AE3) and their cocktail antibody (mixture of them) were used for immunocytochemical stainings. 14 blood specimens were examined (6 stem cell blood specimens, 8 primary advanced ovarian cancer blood spcimens). Results The sensitivity of detection achieved by cocktail antibodies was superior to either COC183B2 or CK MAb alone. The sensitivity of cocktail antibody was 15105. According to the established sensitivity, none of the fourteen specimens contained tumor cell. Conclusions None of the 6 PBSC specimens obtained from 2 patients and eight peripheral blood specimens obtained from 8 patients with primary ovarian epithelial carcinoma contained tumor cell.