河北省2株汉城病毒的遗传特征分析

目的 对河北省2株汉城病毒(SEOV)进行基因分型及系统进化分析.方法 用RT-PCR法扩增SEOV Li株和LF18株的S与M全基因片段;利用DNAStar软件分析S与M基因片段核苷酸序列同源性;利用Phylip软件构建系统进化树.结果 Li株与LFl8株的全S基因片段均由1772个核苷酸组成,开放阅读框(ORF)的位置均为43～1332核苷酸,编码429个氨基酸的核蛋白.2株病毒的M片段全基因序列均由3653个核苷酸组成,ORF的位置均为49～3450核苷酸,编码1133个氨基酸的糖蛋白前体.2株病毒的糖蛋白前体有62个半胱氨酸残基以及6个糖基化位点.2株病毒的全S、M基因片段的核苷酸序列与包括疫苗株L99、Gou3、Z37在内的已知SEOV的同源性分别为87.6%～99.2%和83.6%～97.3%.全S和全M基因片段构建的系统进化树显示2株病毒均为SEOV第Ⅲ亚型.结论 Li株和LF18株都属于SEOV,为SEOV的第Ⅲ亚型.河北省汉坦病毒的流行毒株的遗传特征与疫苗株Z37相似.

Genetic characterization of two Seoul virus strains isolated from patients and rats in Hebei province

Objective Complete S and M segments of two Seoul virus (SEOV) strains were obtained to determine their genetic types and characteristics. Methods The complete S and M segments from the isolate Li and lung tissue (sample LF18) were amplified by RT-PCR. Genetic analyses were performed by using DNAStar and PHYLIP program package. Results Their sequences consisted of 1772 nucleotides, and had an open reading frame (ORF, 43 to 1332 nt)encoding a nucleoprotein of 429 amino acids for both two strains. The complete M segment sequences consisted of 3653 nucleotides and had an ORF encoding a GnGc precursor of 1133 amino acids. The GnGc precursor of the two strains had 62 cysteine and 6 N-glycosylation sites. Both two strains shared a high degree of homology with other known SEOV strains including strains L99, Gou3, and vaccine strain Z37, with 87.6% to 99.2% and 83.6% to 97.3% nucleotide identities, respectively. On the S-and M- trees, the two strains LF18 and Li were grouped into the third cluster of SEOV. Conclusion Both LF18 and Li strains belonged to SEOV and shared the congruent genetic characteristics with the vaccine strains Z37. Thus, the bivalent vaccine including the strain Z37 could effectively prevent HFRS which was caused by SEOV in Hebei province.