HPLC法测定胃炎口服液中黄芩苷的含量

目的:建立以高效液相色谱法测定胃炎口服液中黄芩苷含量的方法。方法:色谱柱为Zorbax SB-C18(150mm×4.6mm,5μm),流动相为甲醇-水-磷酸(50∶50∶0.2),检测波长为276nm。结果:黄芩苷进样量在0.065~0.520μg范围内与峰面积积分值呈良好的线性关系(r=0.9998);平均回收率为99.79%,RSD=1.28%(n=6)。结论:本方法简便、准确,可用于胃炎口服液的质量控制。

Determination of Baicalin in Oral Liquid for Gastritis by HPLC

OBJECTIVE： To establish an HPLC method to determine the content of Baicalin in Oral liquid for gastritis. METHODS： Zorbax SB- C18（150 mm × 4.6 mm, 5μm） was used as chromatographic column, and the mobile phrase consisted of methanol- water- phosphoric acid（50 ： 50 ： 0.2） with detection wavelength set at 276 nm.RESULTS： The calibration curve of baicalin was in good linearity over the range of 0.065--0. 520μg （r = 0.999 8）. The average recovery of Baicalin was 99.79% with RSD at 1.28% （n = 6） .CONCLUSION： The method is simple and accurate and applicable for the quality control of Oral liquid for gastritis.