补阳还五汤对阿尔茨海默病小鼠学习记忆及海马β淀粉样前体蛋白的影响

目的:探讨补阳还五汤对阿尔茨海默病小鼠学习记忆能力及海马β淀粉样前体蛋白(APP)的影响。方法:APP/PS1双转基因小鼠随机分成对照组、模型组、治疗组(吡拉西坦0.62 g·kg-1)、补阳还五汤高剂量组(37.06 g·kg-1)、补阳还五汤中剂量组(18.53 g·kg-1)、补阳还五汤低剂量组(9.26 g·kg-1)。给予补阳还五汤ig,连续治疗28 d后取材。采用Morris水迷宫测试行为学指标,采用双抗体夹心法测定海马组织APP蛋白水平。结果:与对照组比较,模型组小鼠逃避潜伏期延长(P<0.05),游泳距离增加(P<0.05),APP水平增加(P<0.05);与模型组比较,补阳还五汤能改善模型小鼠的学习和记忆(P<0.05),并且降低海马APP水平(P<0.05)。结论:补阳还五汤能改善模型小鼠的学习记忆功能,能够通过抑制APP蛋白在阿尔茨海默病治疗中发挥重要的作用。

Effects of Buyang Huanwu Decoction on Learning and Memory Ability and Amyloid Precursor Protein of Hippocampal in Alzheimer's Disease Mouse

Objective: To study the effects of Buyang Huanwu decoction (BYHWD) on learning and memory ability and amyloid precursor protein (APP) of Hippocampal in Alzheimer's disease mouse. Method: APP/PSI double transgenic mice were randomly divided into control group, model group, treatment group (Piracetam 0.62 g·kg^-1), BYHWD high-dose group(37.06 g·kg^-1), BYHWD medial-dose group(18.53 g·kg^-1), BYHWD low-dose group(9.26 g·kg^-1). The mice were killed after 28 days continuous administration.The Morris water maze was used to observe the learning and memory ability for the mouse. Biochemical methods were used to determine the content of APP protein in the hippocampal tissue. Result: Compared with the control group, model group latencies and swimming distances significantly prolonged(P<0.05), APP significantly increased(P<0.05);compared with the model group, BYHWD could significantly improve the learning and memory ability of model mouse(P<0.05). BYHWD could decrease the content of APP significantly in the hippocampal (P<0.05). Conclusion: BYHWD could improve the ability of learning and memory of model mouse. BYHWD plays a certain role in the treatment of Alzheimer's disease through inhibiting APP.