| Livin、Survivin在人肝癌多药耐药细胞株中的表达及意义 |
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| 引用本文： | 黄涛,杨盛力,尹太勇,曹仕琼,赵志辉,薛磊. Livin、Survivin在人肝癌多药耐药细胞株中的表达及意义[J]. 山东医药, 2011, 51(11): 12-14 |
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| 作者姓名： | 黄涛 杨盛力 尹太勇 曹仕琼 赵志辉 薛磊 |
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| 作者单位： | 1. 武汉科技大学附属天佑医院,武汉,430064
2. 华中科技大学同济医学院附属梨园医院 |
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| 基金项目： | 湖北省科技计划自然科学基金资助项目，武汉科技大学青年科技骨干培育计划项目 |
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| 摘 要： | 目的探讨存活蛋白（Livin）、生存蛋白（Survivin）在人肝癌多药耐药（MDR）细胞株Bel-7402/5-Fu中的表达及其与肝癌MDR的关系。方法通过5-Fu药物浓度梯度递增法诱导建立肝癌耐药细胞亚系Bel-7402/5-Fu,Real-time PCR法检测Bel-7402、Bel-7402/5-Fu中Livin、Survivin mRNA的表达,蛋白印迹法检测Bel-7402、Bel-7402/5-Fu中Livin、Survivin蛋白的表达。结果 Bel-7402/5-Fu细胞中Livin、Survivin mRNA和蛋白的表达量均高于Bel-7402细胞。结论 Livin、Survivin的高表达可能是人肝癌细胞MDR形成的原因之一。
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| 关 键 词： | 存活蛋白 生存蛋白 肝肿瘤 细胞凋亡 多药耐药 |
Expression and significance of Livin and Survivin in multi-drug resistant human hepatocellular carcinoma cell |
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| HUANG Tao,YANG Sheng-li,YIN Tai-yong,CAO Shi-qiong,ZHAO Zhi-hui,XUE Lei. Expression and significance of Livin and Survivin in multi-drug resistant human hepatocellular carcinoma cell[J]. Shandong Medical Journal, 2011, 51(11): 12-14 |
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| Authors: | HUANG Tao YANG Sheng-li YIN Tai-yong CAO Shi-qiong ZHAO Zhi-hui XUE Lei |
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| Affiliation: | HUANG Tao1,YANG Sheng-li,YIN Tai-yong,CAO Shi-qiong,ZHAO Zhi-hui,XUE Lei(1 Tianyou Hospital,Wuhan University of Science and Technology,Wuhan 430064,P.R.China) |
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| Abstract: | Objective To explore the expression of Livin and Survivin protein in multi-drug resistant(MDR)human hepatocellular carcinoma cell line and its relationship with MDR.Methods The hepatocellular carcinoma cell strain Bel-7402 was induced into a multi-drug resistant cell strain stepwise with 5-Fu.The levels of mRNA and protein of Livin and Survivin were detected by Real-time PCR and Western blot in multi-drug resistant human hepatocellular carcinoma cell line Bel-7402/5-Fu and hepatocellular carcinoma cell line... |
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| Keywords: | livin survivin hepatic carcinoma apoptosis multi-drug resistance |
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