海洛因对仔鼠和母鼠肺中SOD、CAT活力和MDA含量及组织结构的影响

目的 探讨海洛因对发育中仔鼠及母鼠肺组织中超氧化物歧化酶(SOD)、过氧化物氢(CAT)活力和丙二醛(MDA)含量及组织结构的影响.方法 对48例受孕小鼠(第8天开始)每天早晚分别注射0.2ml浓度为1.0g/L、1.5g/L和2.0g/L的海洛因溶液和等量的生理盐水,直到小鼠分娩,采用比色法检测胚胎15d、生后1d、7d、15d的仔鼠和母鼠肺中SOD、CAT活力及MDA含量的变化,应用生物显微技术观察各发育期仔鼠和母鼠肺组织结构的变化. 结果 海洛因对各发育期仔鼠和母鼠肺中SOD、CAT活力有显著的抑制作用(P<0.05),且抑制作用随海洛因浓度的增大而加大,但MDA含量则显著增加(P<0.05),海洛因浓度越大仔鼠和母鼠肺中MDA的含量就越高;1.5g/L和2.0g/L海洛因组各发育期仔鼠和母鼠肺泡直径和肺泡隔厚度与对照组相比显著增加(P<0.05).结论海洛因对各发育期仔鼠和母鼠肺有不同程度的损伤作用,这可能与海洛因引起肺组织中SOD、CAT活力下降和MDA含量升高有关.

Effects of heroin on SOD and CAT activity and MDA content and histological structure in the lungs of the filial mice and mother mice

Objective 　To study the effects of enzymatic activity of superoxide dismutase (SOD) and catalase (CAT)，and maleic dialdehyde (MDA) content and histological structure in the lungs of the developing filial mice and mother mice. Methods Totally 48 female mice at the eighth day of pregnancy were injected with different concentrations (1.0g/L，1.5g/L and 2.0g/L) of heroin (0.2ml) and the same amount of saline in the morning and afternoon until the birth of filial mice. Changes in the enzymatic activity of SOD，CAT and MDA content were respectively analyzed by colorimetry and the lung structure of filial mice and mother mice were observed by microscopy at embryo 15 days and postnatal ages of 1day, 7days and 15days. Results There was a significant inhibition for the CAT and SOD activity (EM>P/EM><0.05) following heroin adminstration，and the inhibitive effects were stronger with increasing concentration of heroin increasing; however, the MDA content significantly increased (P<0.05) and was higher with increasing concentrations of heroin. The diameter of the alveolous and thickness of interalveolar septum of filial mice and mother mice were significantly increased (EM>P/EM><0.05) with injection of 1.5g/L or 2.0g/L heroin than that of the controls at the different developmental stages.Conclusion The lungs of filial mice and mother mice at the different developmental stages were damaged in different degrees by heroin. This damage might be correlated with the low activities of SOD, CAT and high cont