Rho激酶抑制剂Y-27632对人U87胶质瘤细胞侵袭能力的影响

目的观察Rho激酶(ROCKS)特异性抑制剂Y-27632对体外培养的U87胶质瘤细胞侵润能力的影响。方法体外培养U87胶质瘤细胞,采用Transwell体外细胞侵润实验,用浓度分别为0、10、25、50μmol/L的Y-27632处理U87细胞,检测下室中洗脱液的吸光度值(OD值)比较不同浓度的Rho激酶抑制剂Y-27632对人U87胶质瘤细胞侵袭能力的影响。实验结果用SPSS 17.0进行方差分析。结果 Transwell小室培养的U87胶质瘤细胞洗脱液的OD值分别为(0.28±0.052)、(0.22±0.046)、(0.17±0.023)、(0.12±0.034)。其中当Y-27632浓度为25μmol/L时其OD值与对照组统计学处理差异有统计学意义(P<0.05);50μmol/L时其OD值与对照组比较,差异有统计学意义(P<0.01);25μmol/L组与50μmol/L组相比,差异有统计学意义(P<0.05)。结论适当浓度下Rho激酶抑制剂Y-27632能明显抑制U87胶质瘤细胞的体外侵润能力。

Effect of Y-27632 Rho-kinase inhibitor on proliferation and invasion of human glioblastoma cell

Objective To explore the effects of Y-27632,inhibitor of Rho-kinase on invasion of U87 glioblastoma cells in vitro. Methods U87 cells were cultured in vitro and then treated with Y-27632 of different concentrations in superior Transwell cabin,cell invasion inhibition abi1ity was detected by the optical density difference(OD) value from eluant of inferior side of Transwell filter membrane.Data were analyzed by SPSS 17.0. Results There was significant difference among invasion of U87 glioblastoma cell treated by Y-27632 with concentration 0,10,25,50 μmol/L,and the OD value was 0.28±0.052,0.22±0.046,0.17±0.023,0.12±0.034.Contrasted with 0/10 μmol/L group,the OD of 25 μmol/L group was significantly low( P <0.05),50 μmol/L group was sharply low( P <0.01). Conclusion Y-27632 could significantly inhibit the invasion ability of U87 glioblastoma cell.