| 乌头碱对心衰细胞强心作用的量-时-效关系的实验研究 |
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| 引用本文： | 温善姗,万海同,张宇燕,杨洁红. 乌头碱对心衰细胞强心作用的量-时-效关系的实验研究[J]. 中国中医急症, 2012, 21(4): 562-564 |
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| 作者姓名： | 温善姗 万海同 张宇燕 杨洁红 |
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| 作者单位： | 浙江中医药大学,浙江杭州,310053 |
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| 基金项目： | 国家自然科学基金项目(No.81073139);高校博点专项基金(No.20113322110002,20093322110001);浙江省中医药科技计划项目(No.2010ZA017) |
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| 摘 要： | 目的观察乌头碱对H2O2构建的心衰细胞模型的强心作用的量-时-效关系。方法利用原代培养的乳鼠心肌细胞,加H2O2构建心衰模型,采用均匀设计,分别加5、10、25、50、100μmol/L的乌头碱作用0.5、1、2、4 h及8 h后测MTT,然后进行验证实验。结果 均匀设计预测的最佳组合为乌头碱50μmol/L作用0.5 h,验证实验也证实如此。结论乌头碱对心衰模型有明显强心作用,其最佳量-时-效组合为50μmol/L的乌头碱作用与心衰模型0.5h。
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| 关 键 词： | 乌头碱 心衰细胞 强心 均匀设计 量-效 时-效 |
Experimental Study of Aconitine on Dose-time-effect Relationship in Cardiac Effect of Heart Failure Cells |
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| WEN Shan-shah , WAN Hai-tong , ZHANG Yu-yan , YANG Jie-hong. Experimental Study of Aconitine on Dose-time-effect Relationship in Cardiac Effect of Heart Failure Cells[J]. Journal of Emergency in Traditional Chinese Medicine, 2012, 21(4): 562-564 |
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| Authors: | WEN Shan-shah WAN Hai-tong ZHANG Yu-yan YANG Jie-hong |
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| Affiliation: | WEN Shan-shan,WAN Hai-tong,ZHANG Yu-yan,et al.Zhejiang Chinese Medical University,Zhejiang,Hangzhou,310053 |
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| Abstract: | Objective:To observe the dose-time-effect relationship in cardiac effect of heart failure cells induced by H2O2.Methods:These heart failure models,establised by H2O2 and myocardial cells of neonatal rats,were analyzed by the uniform design method.These models were divided into several groups which were used respectively by the dose of 5 μmol/L,10 μmol/L,25 μmol/L,50 μmol/L and 100 μmol/L for 0.5 h,1 h,2 h,4 h and 8 h separately.Results:The best predicted combination of uniform design was the 50 μmol/L of aconitine effect for 0.5 h.Meanwhile,the validation experiments also confirmed that.Conclusion:The cardiac effect by aconitine on a cell model of heart failure is significant.The best combination of dose-time is 50 μmol / L and 0.5 h. |
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| Keywords: | Aconitine Heart failure cells Cardiac effect Uniform design Dose-effect Time-effect |
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