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| 表达人癌胚抗原重组痘苗病毒的构建 | |
| 引用本文： | 杨洁,杨太成,罗超权,王晓怀,杨英浩,江悦华,伍新尧. 表达人癌胚抗原重组痘苗病毒的构建[J]. 中山大学学报(医学科学版), 1997, 0(1) |
| 作者姓名： | 杨洁 杨太成 罗超权 王晓怀 杨英浩 江悦华 伍新尧 |
| 作者单位： | 中山医科大学生化教研室，广州军区总医院医学实验科 |
| 基金项目： | 广东省卫生厅“九五”五个一工程项目资助 |
| 摘 要： | 用痘苗病毒作载体，成功构建了高效表达人癌胚抗原（ＣＥＡ）的ＣＥＡ－重组痘苗病毒，用１４３ＴＫ－细胞作为宿主细胞，表达的蛋白质只在细胞膜上能检测到，而胞质内和细胞上清液中均未能检测到，可见所表达的蛋白质保持了其原有特性，再次证实痘苗病毒是真核表达的有效载体。本结果为进一步研究ＣＥＡ－重组痘苗病毒能否激活机体的免疫系统杀伤表达ＣＥＡ的肿瘤提供了物质基础。 |
| 关 键 词： | 痘苗病毒／遗传学；癌胚抗原；基因表达；基因重排 |
CONSTRUCTION OF RECOMBINED VACCINIA VIRUS EXPRESSING HUMAN CEA |
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| Yang Jie Yang Taicheng Luo Chaoquan Wang Xiaohuai Yang Yinghao Jiang Yuehua Wu Xinyao. CONSTRUCTION OF RECOMBINED VACCINIA VIRUS EXPRESSING HUMAN CEA[J]. Journal of Sun Yatsen University(Medical Sciences), 1997, 0(1) | |
| Authors: | Yang Jie Yang Taicheng Luo Chaoquan Wang Xiaohuai Yang Yinghao Jiang Yuehua Wu Xinyao |
| Affiliation: | Yang Jie 1 Yang Taicheng 2 Luo Chaoquan 1 Wang Xiaohuai 2 Yang Yinghao 1 Jiang Yuehua 2 Wu Xinyao 1 |
| Abstract: | By using vaccinia virus as a vector, we have successfully established a CEA vaccinia virus recombinant capable of highly expressing CEA. After infecting 143TK cells with the recombinant, the expressed products could be detected by radio immunoassay technique only in cell membrane fragment but not in cell plasma and culture supernate. It further proved that vaccinia virus is an efficient vector for expressing eukaryotic protein and can maintain the characteristics of the protein. Based on this result, it is possible to study whether CEA vaccinia virus recombinant can activate immuno reactive system in vivo for combatting against tumors expressing CEA. |
| Keywords: | vaccinia virus/genetics carcinoembryonic antigen gene expression gene rearangment |
| 本文献已被 CNKI 等数据库收录！ | |