| 中药增骨Ⅰ号对体外成纤维细胞的影响 |
| |
| 引用本文： | 魏玉玲,邹庆,梁克玉.中药增骨Ⅰ号对体外成纤维细胞的影响[J].中医正骨,2004,16(1):13-14. |
| |
| 作者姓名： | 魏玉玲 邹庆 梁克玉 |
| |
| 作者单位： | 湖北省中医院,武汉,430061 |
| |
| 摘 要： | 为了探讨增骨Ⅰ号的作用机制,进行了体外成纤维细胞的实验.采用酶消化法将人包皮组织进行成纤维细胞的分离, 纯化及培养,并测定骨钙素、细胞钙含量.结果显示成纤维细胞在增骨Ⅰ号组增殖迅速,其OD值明显高于对照组(P＜0.05),但反映成骨表型表达的骨钙素及细胞钙含量未见明显增加.表明增骨Ⅰ号仅有激活成纤维细胞的作用,本实验结果为序贯疗法应用增骨Ⅰ号作为骨重建中的激活药物提供了理论依据.
|
| 关 键 词： | 包皮/细胞学 成纤维细胞/培养 重组人骨形态发生蛋白-2 增骨Ⅰ号 骨重建 实验研究 |
| 文章编号： | 1001-6015(2004)01-0013-02 |
THE INFLUENCE OF "ZENG GU" No.1 ON FIBROBLASTS IN VITRO |
| |
| Wei Yuling,Zhou Qing,and Liang Keyu TCM Hospital of Hubei Province,Wuhan.THE INFLUENCE OF "ZENG GU" No.1 ON FIBROBLASTS IN VITRO[J].The Journal of Traditional Chinese Orthopedics and Traumatology,2004,16(1):13-14. |
| |
| Authors: | Wei Yuling Zhou Qing and Liang Keyu TCM Hospital of Hubei Province Wuhan |
| |
| Institution: | Wei Yuling,Zhou Qing,and Liang Keyu TCM Hospital of Hubei Province,Wuhan 430061 |
| |
| Abstract: | In order to explore the action mechanism of "ZENG GU" No. 1 (ZG No. 1, a TCD preparation), fibroblasts (FBS) were experimented in vitro: isolated, purified and cultivated from human foreskin with trypsinase digestion method; and the osteocalcin (BGP) and calcium content in FBS were determined. The results showed that the FBS proliferation in ZG No. 1 group was rapid with OD value obviously higher than that in the control group (P < 0.05) but no significant increase was found in the BGP and calcium content reflecting the osteogenic phenotypic expression, suggesting that ZG No. 1 functions only to activate FBS; the results of this study could provide a theoretic basis on which ZG No. 1 may be used as the activating drug for bone remoulding in the sequential therapy. |
| |
| Keywords: | foreskin/cytolody fibroblasts/culture rhBMP-2 "ZENG GU" No 1 bone remoulding experimental study |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
