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| 反胶束体系中猪胰脂肪酶水解橄榄油的动力学研究 | |
| 引用本文： | 朱宁,郭军,陈润杰,莫凤奎.反胶束体系中猪胰脂肪酶水解橄榄油的动力学研究[J].中国药业,2002,11(8):28-29. |
| 作者姓名： | 朱宁 郭军 陈润杰 莫凤奎 |
| 作者单位： | 1. 深圳市清华源兴药业公司研究发展部,广东,深圳,518057 2. 深圳市创益生物科技有限公司,广东,深圳,518067 3. 沈阳药科大学物理化学教研室,辽宁,沈阳,110015 |
| 摘 要： | 目的：系统研究猪胰脂肪酶在二（2-乙基已基）丁二酸酯磺酸钠（AOT）/异辛烷反胶束体系中水解橄榄油的动力学行为。方法：考察温度，wo(H2O]/AOT])，pH和表面活性剂的浓度对酶反应的影响以及酶的稳定性。结果：酶反应符合常规的一级反应动力不模型，活化有Ea=43.68kJ/mol；最佳pH值为7.7。结论：高浓度表面活性剂对酶表现非竞争性抑制作用；wo是决定酶稳定性的关键。 |
| 关 键 词： | 反胶束 猪胰脂肪酶 橄榄油 动力学 反胶束酶催化 |
| 文章编号： | 1006-4931(2002)08-0028-02 |
| 修稿时间： | 2002年6月21日 |
Kinetic Study of Porcine Pancreatic Lipase-catalyzed Hydrolysis of Olive Oil in AOT/isooctane Reversed Micelles |
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| Zhu Ning,Guo Jun,Chen Runjie,Mo Fengkui.Kinetic Study of Porcine Pancreatic Lipase-catalyzed Hydrolysis of Olive Oil in AOT/isooctane Reversed Micelles[J].China Pharmaceuticals,2002,11(8):28-29. | |
| Authors: | Zhu Ning Guo Jun Chen Runjie Mo Fengkui |
| Abstract: | Subject: Kinetics of olive oil hydrolysis by porcine pancreatic lipase in bis(2-ethylhexyl) sodium sulfos-uccinata(AOT)/isooctane reversed micellar system was systematically studied. Methods: The effect of temperature, pH, water content wo( H2O]/AOT] )and surfactant concentration on lipase activity and the stability has been investigated in detail. Rsults: It was indicated that the hydrolysis reaction follows the first - order kinetic model, Ea=44.00kJ/ mol; optimum pH is 7.7. Conclution: Higher concentration of surfactant showed a non - competitive inhibition on the lipase activity; water content wo is the key factor that affects enzyme stability. |
| Keywords: | Reversed micelle Lipase Olive oil Hydrolysis Kinetics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |