胰岛素样生长因子-1(IGF-1)对成骨细胞的成骨影响

目的：探讨不同浓度IGF－1对兔成骨细胞的成骨影响。方法：采用组织块细胞培养技术获得兔成骨细胞，第2代成骨细胞分别在含0.1ng/ml,1ng/ml,10ng/ml,20ng/mlIGF－1的DMEM中培养，24，36h后行MTT法检测细胞增殖情况，72，108h收集培养上清进行骨钙素放射免疫法（RI）测定。结果：IGF－1与成骨细胞培养24，36h，经MTT法检测，不同浓度IGF－1与对照组比较，有显著性差异（P＜0．001）；IGF－1浓度为0．1、1、10ng/ml，各组之间相比存在显著性差异（P＜0．05）；IGF－1与成骨细胞培养72．108h，经RI检测，不同浓度IGF－1对成骨细胞合成骨钙素与对照组比较无显著性差异（P＞0．05）：结论：IGF－1对成骨细胞有明显促进增殖作用，在0．1－10ng/ml范围，存在浓度依赖性，未发现IGF－1对成骨细胞合成骨钙素有影响。

Effects of insulin-like Growth Factor-1(IGF-1) on Bone Formation of Rabbit Osteoblast in vitro

Objective: To investigate the effects of IGF-1 on bone formation of rabbit osteoblast in vitro.Methods:Osteoblasts were obtained from rabbit costal bone by explant culture technique.The second subculture was exposed to IGF-1 at variant concentrations of 0.1,1,10 and 20ng/ml for 24 and 36 hours,and the effects of IGF-1 on osteoblast proliferation was observed by MTT assay.The contents of BGP were measured by the radioimmunoassay in the culture supernatant harvested after the second subculture were exposed to IGF-1 for 72 and 108 hours.Results:Osteoblast exposed to IGF-1 for 24 and 36 hours at variant concentrations of 0.1,1,10,20ng/ml proliferated significantly compared with the control (P<0.001),and significant differences were found between every two groups when osteoblast were exposed to IGF-1 at range from 0.1 to 10ng/ml(P<0.05).Levels of osteocalcin in culture supernatant of osteoblast exposed to IGF-1 at variant concentrations for 72 and 108 hours was not statistically significant compared with control group (P>0.05).Conclusions:IGF-1 could promote the proliferation of osteoblast,and there were dose-dependent relationship at a limited range from 0.1 to 10ng/ml.IGF-1 had no effect on osteocalcin synthesis of osteoblast.