安心颗粒对慢性心衰大鼠细胞因子和心肌重构的影响

目的观察安心颗粒对慢性心力衰竭(CHF)模型大鼠细胞因子和心肌重构的影响。方法将50只雌性SD大鼠随机分为正常对照组(A组)、模型组(B组)、卡托普利组(C组)、安心颗粒低剂量组(D组)、安心颗粒高剂量组(E组),每组10只。B～E组采用阿霉素腹腔注射造模,每周1次,连续6周。造模同时,C～E组分别按6.25 mg.kg-1.d-1,0.8 g.kg-1.d-1,1.6 g.kg-1.d-1剂量灌胃给药。造模结束后,分别测定各组血清肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)水平及左室重量指数(LVMI)。结果模型组血清TNF-α、IL-6水平及LVMI均显著性升高(P<0.01);安心颗粒低、高剂量组均可显著性降低模型大鼠血清TNF-α、IL-6水平及LVMI(P<0.05或P<0.01),且呈现一定剂量效应关系。结论安心颗粒可通过调控炎性细胞因子、抑制或延缓心肌重构,防治心力衰竭。

Effect of Anxin Granules on Cytokine in Serum and Myocardial Remodeling in Rats withHeart Failure

ObjectiveTo study the effect of Anxin granules on cytokines and myocardical remodeling in rats with heart faliure.MethodsFifty female SD rats were randomly divided into five groups:normal control group(A),model group(B),captopril group(C),low-dosage AXG group(D) and high-dosage AXG group(E).CHF model was established by intraperitoneal injection of low-dose of adriamycin,once a week for continuous 6 weeks.Meanwhile,group C was given captopril injection(6.25 mg·kg-1·d-1),and groups D and E received gastric gavage of low-dosage AXG(0.8 g·kg-1·d-1) and high-dosage AXG(1.6 g·kg-1·d-1) respectively.After 6 weeks of modeling,serum levels of TNF-α and IL-6 in different groups were tested by ELISA,and the left ventricular mass index(LVMI) was measured.ResultsCompared with group A,the serum levels of TNF-α and IL-6,and LVMI were markedly increased in model group(P<0.01).Low-and high-dosage AXG decreased the serum levels of TNF-α and IL-6 as well as LVMI of model rats(P<0.05 or P<0.01),in a dose-dependent manner.ConclusionAXG can alleviate the ventricular remodeling in CHF by regulating the inflammatory factors,restrain the development of CHF,and serves as a remedy for prevention and treatment of CHF.