| 三氧化二砷联合TRAIL抑制肺癌A549细胞增殖及诱导凋亡的研究 |
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| 引用本文： | 王绩英,赵雪强,王昌明,莫碧文,蒋明,陈峰.三氧化二砷联合TRAIL抑制肺癌A549细胞增殖及诱导凋亡的研究[J].时珍国医国药,2011,22(7):1618-1619. |
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| 作者姓名： | 王绩英 赵雪强 王昌明 莫碧文 蒋明 陈峰 |
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| 作者单位： | 桂林医学院附属医院,广西,桂林,541001 |
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| 基金项目： | 广西壮族自治区科技厅自然科学基金，广西壮族自治区卫生厅自筹项目 |
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| 摘 要： | 目的研究三氧化二砷(As2O3)联合肿瘤坏死因子相关凋亡诱导配体(TRAIL)抑制肺癌A549细胞增殖及诱导凋亡的作用。方法采用不同浓度的As2O3与TRAIL联合作用于体外培养的肺癌A549细胞,以四甲基偶氮唑蓝(MTT)比色法和Hoechest染色观察检测其对A549细胞的抑制作用。以流式细胞术检测其对A549细胞的凋亡诱导作用和细胞周期。结果不同浓度的As2O3与TRAIL联用对A549细胞增殖的抑制作用均比单药组强。联合用药组从形态学观察及流式细胞术结果显示联合用药组可检测到大量凋亡A549细胞,明显多于单药组;并将A549细胞阻滞在G2/M期。结论 As2O3与TRAIL联用于人肺癌A549细胞可协同抑制其增殖,机制可能是将细胞阻滞在G2/M期;并可诱导细胞凋亡。
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| 关 键 词： | 三氧化二砷 肿瘤坏死因子相关凋亡诱导配体 肺肿瘤 凋亡 增殖 |
Proliferation and Apoptosis of Human Lung Cancer Call Line A549 |
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| WANG Ji-ying,ZHAO Xue-qiang,WANG Chang-ming,MO Bi-wen,JIANG Ming,CHEN Feng.Proliferation and Apoptosis of Human Lung Cancer Call Line A549[J].Lishizhen Medicine and Materia Medica Research,2011,22(7):1618-1619. |
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| Authors: | WANG Ji-ying ZHAO Xue-qiang WANG Chang-ming MO Bi-wen JIANG Ming CHEN Feng |
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| Institution: | WANG Ji-ying,ZHAO Xue-qiang,WANG Chang-ming,MO Bi-wen,JIANG Ming,CHEN Feng(The Affiliated Hospital of Guilin Medical College,Guilin 541001,China) |
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| Abstract: | ObjectiveTo study the effect of As2O3 and TRAIL on A549 cell proliferation and apoptosis.MethodsHuman lung cancer cell line A549 was treated with As2O3 and TRAIL at different concentrations in vitro.The inhibitory effect was observed by MTT assay.Hoechest staining was applied to analyze the apoptosis effect.Flow cytometry with PI staining was used to detect the apoptosis rate and cell cycle after treatment.ResultsDifferent concentrations of As2O3 combined TRAIL had more stronger inhibitory effect on A549 ce... |
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| Keywords: | Arsenic trioxide Tumor necrosis factor related apoptosis inducing ligand Lung neoplasm Apoptosis Proliferation |
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