侧脑室注射甘珀酸对扩散性抑制的影响

目的:探讨缝隙连接蛋白阻断剂甘珀酸(CBX)对扩散性抑制(SD)的影响。方法:SD大鼠30只,随机分为对照组、假手术组和CBX干预组各10只,后两者用高浓度KCl诱导大鼠皮质SD波,假手术组不给予CBX干预,CBX干预组给予5 ug/uL的CBX进行干预,用MS4000U生物信号采集分析系统对波幅、时程进行计算分析。结果:与假手术组比较,CBX干预组所记录到SD波波幅明显减小(P<0.05),SD波的阳性率降低(P<0.05),但SD波的时程无差异。结论:CBX对高浓度KCl诱导的皮质SD波有明显的抑制作用,提示缝隙连接在SD的发生发展过程中具有重要作用。

Preliminary Observation of the Effects of Carbenoxolone on Spreading Depression

Objective: The effect of gap junction protein inhibitor Carbenoxolone(CBX) on spreading depression(SD) has been observed.Methods: The rats were divided into three groups,the control group without intervention,sham-operation group,and the CBX treated group.In the later two groups,cortex SD waves were generated by using high concentrated KCl.In CBX intervention group,5ug/μL CBX were given.The MS4000U biology signal gathering analysis system was employed to collect and analyze the amplitudes and durations of SD waves.Results: The amplitudes of SD waves in the CBX intervention group were significantly reduced when compared to the control group(P<0.05).Meanwhile,the positive rate of SD waves was also decreased significantly(P< 0.05).However,no significant difference in the durations of SD waves was found between the CBX intervention group and control group(P> 0.05).Conclusion: Gap junction inhibitor CBX is effective to restrain cortex SD induced by high concentrated KCl,suggesting the role of gap junction in the development of SD.