肾康宁片中黄芪甲苷的薄层扫描法测定

目的:建立肾康宁片中黄芪甲甙含量测定方法。方法:双波长薄层扫描法,经水饱和正丁醇液冷浸超声提取,再上D101大树脂纯化,以氯仿-甲醇-水(65:30:10)下层液为展开剂,检测波长为520nm,参比波长为700nm。结果:平均加样回收率为97.8％(RSD=1.4％,n=6),标准曲线r=0.9996。结论:方法结果可靠、操作简单,可作为肾康宁片的质量控制标准。

Quantitative determination of astragaloside Ⅳ in Shenkangning Tablet by TLCS

OBJECTIVE:To establish a method for content determination of astragaloside IV in Shekangnin TABLET. METHODS:Using dual - wavelength TLC - scanning,the samples were extracted by n - butanol saturated with water in an ultrasonator and pured by macroporous resin,the mobile phase is chloroform - methanol - water(65 : 30: 10) (the lower) , detection wavelength is 520nm,reference wavelength is 700nm.RESULTS:The average recovery is 97.8%(RSD = 1.4% , n=6),with a good linear relationship (r = 0.9996) .CONCLUSION: The method is reliabe and simple and can be used for the quality control of Shenkangning tablet.