槲皮素减轻高糖条件下人肾小球内皮细胞损伤的实验研究

目的研究槲皮素减轻高糖条件下人肾小球内皮细胞(HRGEC)损伤的作用及其机制。方法培养HRGEC并分为对照组、高糖组、10μmol/L槲皮素组、100μmol/L槲皮素组,后3组进行高糖处理,10μmol/L及100μmol/L槲皮素组分别给予10μmol/L及100μmol/L槲皮素处理。检测细胞活力、细胞凋亡、凋亡基因mRNA表达量、氧化应激指标含量、PI3K/AKT通路分子蛋白表达量。结果与对照组比较,高糖组的细胞活力、Bcl-2的mRNA表达量、SOD及GPx的含量、p-PI3K及p-AKT的蛋白表达量降低,细胞凋亡率、Bax及Caspase-3的mRNA表达量、MDA的含量增加(P<0.05);与高糖组比较,10μmol/L及100μmol/L槲皮素组的细胞活力、Bcl-2的mRNA表达量、SOD及GPx的含量、p-PI3K及p-AKT的蛋白表达量增加,细胞凋亡率、Bax及Caspase-3的mRNA表达量、MDA的含量降低(P<0.05)且100μmol/L槲皮素组的上述变化较10μmol/L槲皮素组更为显著(P<0.05)。结论槲皮素能够减轻高糖条件下HRGEC的损伤,且这一作用与激活PI3K/AKT通路有关。

Experimental study on quercetin reducing the injury of human glomerular endothelial cells under high glucose condition

Objective To study the effect of quercetin on reducing the injury of human glomerular endothelial cell(HRGEC) under high glucose condition.Methods HRGEC was cultured and divided into control group,high glucose group,10μmol/L and 100μmol/L quercetin group.The latter three groups were treated with high glucose.10μmol/L and 100μmol/L quercetin groups were treated with 10μmol/L and 100μmol/L quercetin respectively.Cell viability,apoptosis,mRNA expression of apoptotic gene,contents of oxidative stress indexes and protein expression of PI3 K/Akt pathway were measured.Results Compared with the control group,the cell viability,bcl-2 mRNA expression,SOD and GPX contents,p-PI3 K and p-AKT protein expression decreased,apoptosis rate,Bax and caspase-3 mRNA expression,MDA content increased in the high glucose group(P <0.05).Compared with the high glucose group,the cell viability,bcl-2 mRNA expression,SOD and GPX contents,p-PI3 K and p-AKT protein expression increased,apoptosis rate,Bax and caspase-3 mRNA expression,MDA content decreased in 10μmol/L and 100μmol/L quercetin groups(P <0.05) and the above changes of 100μmol/L quercetin group were more significant than those of 10μmol/L quercetin group(P <0.05).Conclusion Quercetin can reduce the damage of HRGEC under high glucose condition,and this effect is related to activation of PI3 K/Akt pathway.