响应面分析法优化回流提取延龄草甾体皂苷工艺研究

目的通过响应面法优化延龄草中3种甾体皂苷的提取工艺。方法采用HPLC法,以延龄草中重楼皂苷Ⅵ(Y2T)、偏诺皂苷元-3-O-α-L-吡喃鼠李糖基(1→4)-[α-L-吡喃鼠李糖基(1→2)]-β-D-吡喃葡萄糖苷(Y3T)、重楼皂苷Ⅶ(Y4T)得率为评价指标,通过单因素试验,考察了提取时间、提取次数、液料比对3种甾体皂苷提取效果的影响,并采用Box-Behnken设计-响应面法分析,优选最佳提取工艺。结果最佳提取工艺为70%乙醇,提取时间为120 min,提取3次,液料比为15:1。结论响应面法优化的延龄草3种甾体皂苷提取工艺,稳定、可行。

Optimization of extraction of steroidal saponins from Trillium tschonoskii Maxim.Using Response Surface Methodology

Objective To optimize the extraction process of three kinds of steroidal saponins from Trillium tschonoskii Maxim.Methods The method of HPLC was used to determine the content of paris saponin(Y2T),Pinosaponin-3-O-α-L-rhamnopyranoside(1→4)-[α-L-rhamnopyranoside(1→2)]-β-D-glucopyranoside(Y3T),paris saponinⅦ(Y4T).The effects of extraction time,extraction times and ratio of material to liquid on the extraction of three steroidal saponins were investigated by single factor experiment.The optimum extraction process was selected by Box-Behnken design-response surface method.Results The optimum extraction technology was 70%ethanol,the extraction time was 120 min,and the ratio of material to liquid was 15:1.Conclusion The three kinds of steroidal saponins extraction process optimized by response surface method is stable and feasible.