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| MTT检测超声激活血卟啉对SW-480细胞的杀伤作用 | |
| 引用本文： | 张坤,刘全宏,齐浩,张军平,李清,李萌. MTT检测超声激活血卟啉对SW-480细胞的杀伤作用[J]. 中国医学生物技术应用, 2003, 0(1) |
| 作者姓名： | 张坤 刘全宏 齐浩 张军平 李清 李萌 |
| 作者单位： | 陕西师范大学生命科学学院 西安710062(张坤，刘全宏，齐浩，李清)，陕西师范大学应用声学研究所 西安710062(张军平)，陕西师范大学生命科学学院 西安710062(李萌) |
| 基金项目： | 国家自然科学基金资助项目基金编号:39870240 |
| 摘 要： | 本实验采用1.1、1.6、2.3MHz频率聚焦超声结合血卟啉的方式对体外培养的人结肠癌细胞SW-480进行照射,其介质分别为NaCl溶液、PRMI1640、完全培养基,并采用MTT法测试SDT对肿瘤细胞的杀伤效果。结果表明:SW-480细胞在声照强度为1.5W/cm2 ,频率2.3 MHz条件下,介质为NaCl溶液、PRMI1640和完全培养基中都存在超声激活血卟啉增强杀伤肿瘤细胞的效果,且在PRMI1640和完全培养基中这种效果和处理后细胞孵育的时间有一定的相关性。 |
| 关 键 词： | MTT 聚焦超声 血卟啉 SW-480细胞系 |
Effects of Hematoporphyrin Activated with Lower Intensity Focus Ultrasound on SW-480 Cells Measured by MTT Colorimetric Assay |
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| Zhang Kun,Liu Quan-hong,Qi Hao,Zhang Jun-ping,Li Qing,Li Meng. Effects of Hematoporphyrin Activated with Lower Intensity Focus Ultrasound on SW-480 Cells Measured by MTT Colorimetric Assay[J]. The Chinese Academic Medical Magazine of Organisms, 2003, 0(1) | |
| Authors: | Zhang Kun Liu Quan-hong Qi Hao Zhang Jun-ping Li Qing Li Meng |
| Affiliation: | Zhang Kun1,Liu Quan-hong1,Qi Hao1,Zhang Jun-ping2,Li Qing1,Li Meng11 |
| Abstract: | SW-480 cells with hematoporphyrin were exposed to focus ultrasound of different frequency (1.1MHz, 1.6 MHz, 2.0 MHz, 2.3 MHz) and the same intensity of 1.5W/cm2. The cell lysis was examined by MTT assay. Theexperiment results indicated that SW-480 cells with hematoporphyrin was remarkably killed by hematopophyrinactivated with focus ultrasound of frequency of 2.3 MHz. But this effect was not found in SW-480 cells withouthematoporphyrin.[ |
| Keywords: | MTT Focus ultrasound HPD SW-480 cell line |
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