江西地方药材野山楂叶质量标准研究

目的：建立江西地方药材野山楂叶的质量标准.方法：建立药材的性状、显微特征和熊果酸TLC鉴别方法;采用HPLC方法测定熊果酸含量,色谱柱为Thermo BDS HYPERSIL C18（250 mm×4.6mm,5μm）,以甲醇-0.05％磷酸（87∶ 13）为流动相,流速为1.0 mL/min,检测波长为210nm.结果：山楂叶的显微特征性强;熊果酸TLC色谱斑点清晰;熊果酸在0.01984～0.1984mg范围内呈良好线性关系,r=0.9991,回收率为98.71％,RSD为0.64％（n=3）.结论：方法简便,准确,重复性好,适用于江西地方药材野山楂叶的质量控制.

Study on the Quality Standard of Jiangxi Local Herb of Hawthorn Leaves

Objective： To establish the quality standard for wild hawthorn leaves. Methods： To establish properties, microscopic characteristics, and ursolic acid TLC identification of traditional Chinese herbs; Content determination of ursolic acid by the method of HPLC, the column was Thermo BDS HYPERSIL C18 （250mm × 4.6mm, 5μm）, using methanol -0.05% phosphate （87：13 ） as mobile phase, the flow rate was 1.0 mL / min, the detection wavelength was 210 nm. Results： The wild hawthorn leaf microstructure had strong characteristics; ursolic acid TLC chromatographic spots were clear; ursolic acid ranged in 0.01984 - 0. 1984 mg showed good linear relationship in the range, the correlation coefficient was 0. 9991. The recovery rate was 98.71% with RSD being 0.64%. （n = 9）. Conclusion： The method is simple, accurate, reproducible, and suitable for the quality control of wild hawthorn leaves.