Assignment of mouse beta-spectrin gene to chromosome 12 |
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| Authors: | Pekka Laurila Livia Cioe Christine A. Kozak Peter J. Curtis |
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| Affiliation: | (1) The Wistar Institute of Anatomy and Biology, 36 and Spruce Streets, 19104 Philadelphia, Pennsylvania;(2) Present address: Institute of Virology, University of Rome, 00185 Rome, Italy;(3) Laboratory of Molecular Biology, National Institute of Allergy and Infectious Diseases, 20892 Bethesda, Maryland |
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| Abstract: | ![]()
The structural gene for the Β-subunit of the mouse erythrocyte spectrin, hereinafter designated as Sp-b, was assigned to the mouse chromosome 12. This assignment was made by Southern analysis of genomic DNA from mouse X Chinese hamster hybrid cells using cloned mouse erythrocyte Β-spectrin cDNA as a probe. In the PstI-digested genomic hamster cell DNA a single band of 2.0 kb was detected, whereas PstI-digested mouse DNA gave a band of 4.2 kb, when probed with the mouse erythroid Β-spectrin cDNA clone. This allowed us to analyze a panel of mouse X Chinese hamster somatic cell hybrids to map this gene to chromosome 12. Interestingly, this assignment is different from that observed for the α-subunit of spectrin, which has been mapped to chromosome 1 in mouse. These results serve as a basis for further genetic characterization of the mouse hemolytic anemias. |
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