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蒺藜总皂苷含量测定方法研究
引用本文:李瑞海,杨松松.蒺藜总皂苷含量测定方法研究[J].实用药物与临床,2006,9(2):77-78.
作者姓名:李瑞海  杨松松
作者单位:辽宁中医学院,沈阳,110032
摘    要:目的建立蒺藜总皂苷的含量测定方法。方法采用分光光度法,选用菝葜皂苷元(Sarsasa- pogenin)为对照品,通过高氯酸显色,计算蒺藜药材中总皂苷的含量。结果菝葜皂苷元在0.230 4-0.720 0 mg 范围内具有良好的线性关系(γ=0.999 3),平均回收率为98.54%,RSD为1.57%。结论该方法简便、准确、灵敏,重现性良好,适用于蒺藜总皂苷的含量测定。

关 键 词:蒺藜  菝葜皂苷元  总皂苷  分光光度法
文章编号:1673-0070(2006)02-0077-02
收稿时间:2005-12-26
修稿时间:2005年12月26

Determination of the content of total saponins in tribulus terrestis L
LI Rui-hai,YANG Song-song.Determination of the content of total saponins in tribulus terrestis L[J].Practical Pharmacy and Clinical Remedies,2006,9(2):77-78.
Authors:LI Rui-hai  YANG Song-song
Abstract:Objective To determine the content of total saponins in Tribulus terrestis L. Methods UV spectral method was used. The samples or Sarsasapogenin reacted with the perchloric acid. Results There was good lineari-ty( r = 0.999 3 ) during the range 0.230 4 - 0.720 0mg. The average recovery was 98.54 %, RSD was 1.57%. Conclusion The method is simple, sensitive and accurate, and it can be used as a quantitative analytical method for Tribulus terrestis L.
Keywords:Tribulus terrestris L  Sarsasapogenin  Total saponins  UV-spectral method
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