抑颤汤治疗帕金森病的实验研究

目的：探讨抑颤汤治疗帕金森病（Parkinson′s disease,PD）的作用机制。方法：建立PD大鼠模型，设立正常对照组、生理盐水对照组、抑颤汤组，观察抑颤汤治疗前后PD大鼠行为特征、脑黑质神经细胞变化；采用脑组织液微透析技术，用高效液相色谱法测定细胞外液中儿茶酚胺类物质含量的变化；用放免法测定M、DA受体数量及亲和力的变化。结果：抑颤汤能明显改善PD模型大鼠的旋转行为，同时提高了损毁侧脑组织DT和M爱体最大结合量（Bmax）和亲和常数（KD），尾状核和壳状核部位的细胞外液中DOPAC、HVA、DA、5－HT水平明显低于未损毁侧，与生理盐水组对照差异显著（P＜0．01）。与生理盐水组对照，脑黑质神经细胞数量明显增多，神经元体积较饱满，结构较清晰，细胞内高尔基体、线粒体等趋于正常。结论：抑颤汤能刺激PD模型大鼠受体系统，使DA、M受体的亲和力和敏感性提高；促进其损毁侧黑质纹状体分泌儿茶酚胺类物质，提高脑组织中儿茶酚胺类物质的含量；促进受损脑黑质神经细胞的修复，为临床应用抑颤汤治疗PD提供了实验依据。

Experimental research on the treatment of Parkinson''s disease with Yichantang

Objective:To explore the mechanism of action in the treatment of Parkinson's disease (PD) with Yichantang. Methods: A PD rat model was made and a normal control group, a normal saline control group and a Yichantang group were set up to observe changes in the rats' behavior pattern and the cerebral black substance cells. Using the technique of intracerebral dialysis, the content of monoamine substances in the ECF was measured by means of HPL and changes in the affinity and quantity of M and DA receptors were measured. Results: Yichantang markedly improved the rats' rotation while enhancing the Bmax and KD of DA and M receptors in the brain tissues on the lesioned side. The levels of DOPAC, HVA, DA and 5-HT in the ECF of caudate nucleus and shell nucleus on the lesioned side were significantly lower than those on the unlesioned side. The treatment resulted in a marked difference from the normal saline group (P<0.01). Besides, the quantity of cerebral black substance cells significantly increased and the volume and structure of the neurons and intracellular Golgi complex and mitochondria were close to normal. Conclusion: Yichantang can increase the affinity and sensitivity of DA and M receptors by stimulating the receptor system of the PD model rats, help the striate body of the black substance on the lesioned side to secret CA substances, and facilitate the repair of the lesioned black substance cells, providing experimental basis for the clinical treatment of PD.