Comparison of the performance of enzyme immunoassays for hepatitis B and C detection in dried blood spot |
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| Authors: | Helena Medina Cruz Juliana Custódio Miguel Cruz Elisangela Ferreira Da Silva Moyra Machado Portilho Vanessa Alves Marques Lia Laura Lewis-Ximenez |
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| Affiliation: | Laboratory of Viral Hepatitis, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil |
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| Abstract: | Dried blood spots (DBSs) could be an alternative to serum for hepatitis B virus (HBV) and hepatitis C virus (HCV) diagnosis. This study aims to evaluate two enzyme immunoassays (EIAs) for HBsAg and anti-HCV detection using DBS. Serum was tested using commercial EIA. DBS was tested using optimized EIA developed for serum and commercial EIA developed for DBS (Imunoscreen). Concordances between DBS and serum samples for both markers and EIAs were higher than 97%. Both EIAs demonstrated good performance for HBsAg and anti-HCV detection using DBS, and these methods could be used unchangeably increasing the access for HBV and HCV diagnosis. |
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| Keywords: | dried blood spots hepatitis B hepatitis C enzyme immunoassays |
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