抗凋亡蛋白BAG-1在非小细胞肺癌组织中的表达及其与细胞凋亡、Bcl-2蛋白表达的关系

目的研究非小细胞肺癌组织中抗凋亡蛋白BAG-1的表达和临床意义,并探讨其与癌细胞凋亡及Bcl-2蛋白表达的关系。方法采用免疫组织化学SP法和TNUEL法分别检测54例非小细胞肺癌组织中BAG-1、Bcl-2蛋白的表达及其细胞凋亡指数,并检测20例正常支气管黏膜组织中BAG-1蛋白的表达作对照。结果非小细胞肺癌BAG-1蛋白表达阳性率为74.07%,明显高于正常支气管黏膜(阳性率为5%)。其表达与非小细胞肺癌组织分化程度、有无淋巴结转移、TNM分期密切相关,而与性别、年龄、病理类型无关。非小细胞肺癌组织中BAG-1蛋白表达与Bcl-2蛋白表达呈正相关关系(Pearson列联系数为0.406,P<0.01)。随BAG-1蛋白表达强度的增强,癌细胞凋亡指数逐渐减少,各组间有统计学意义。结论BAG-1蛋白在非小细胞肺癌组织中表达显著高于正常肺组织,其表达与非小细胞肺癌发生、发展密切相关,与Bcl-2蛋白表达呈正相关,能抑制癌细胞凋亡,可作为肺癌治疗的新靶点。

THE EXPRESSION OF ANTY-APOPTOSIS PROTEIN BAG-1 IN NON-SMALL CELL LUNG CANCER AND ITS RELATIONSHIP WITH CELL APOPTOSIS AND EXPRESSION OF BCL-2

Objective To study the expression and clinical significance of anty-apoptosis protein BAG-1 in non-small cell lung cancer(NSCLC),and its relationship with cell apoptosis and expression of Bcl-2 protein. Methods Immunohisto chemistry streptavidin-peroxidase conjugated (SP)method was used to examine the expression of BAG-1protein and Bcl-2 protein,and terminal deoxynucleotidyl transferase mediated UTP nick end labeling(TUNEL) method was used to examine the apoptosis index in 54 cases of NSCLC.The expression of BAG-1 protein in 20 cases of normal bronchus mucosa tissue also be detected as control. Results Express positive rate of BAG-1 protein in NSCLC is 74.07%,obviously higher than that in normal bronchus mucosa tissue(positive rate is 5%).In cases of NSCLC,the expression of BAG-1 protein has not correlation with the age,gender,pathologic classification,but have closed correlation with lymph node metastasis,degree of differentiation,pTNM stage(P<0.05).There is positive correlation betweened BAG-1 protein expression and Bcl-2 protein expression(pearson contingency coefficient is 0.406,P=0.003,P<0.01).Following the increasing of expression degree of BAG-1protein,the apoptosis index of cancer cell was degraded.The statistically significant difference was found between each groups.Conclusion BAG-1 protein is over expression in NSCLC compare with normal bronchus mucosa tissue,have closed relation with NSCLC-genesis and development.There is positive correlation relationship was found between the expression of BAG-1 protein and Bcl-2 protein.It can inhibit cell apoptosis,and can be as a new therapic target for NSCLC.