抗阻训练对老龄大鼠骨骼肌新生细胞核数量的影响

目的:观察10周抗阻训练后老龄大鼠骨骼肌内细胞核数量的变化情况,及其合成能力加强。方法:选取17月龄的SD大鼠40只,随机分为安静组、大、中、小负重训练组,每组10只,用5-溴脱氧尿嘧啶核苷(Br-du)对新生细胞核进行标记,通过免疫组织化学的方法对新生肌核的数量进行比较。结果:新生肌核数量方面:大负荷组(7.5±4.2)和中负荷组(6.7±3.6),显著高于安静组(2.1±1.3)和小负荷组(5.1±2.6)。肌纤维内肌核平均数量大负荷组(3.2±0.6)和中负荷组(3.1±0.5),显著高于安静组(2.4±0.5)和小负荷组(2.8±0.6)。肌肉重量方面,中负荷的(10.6±1.3g)和大负荷(10.1±1.4g),显著高于安静组(8.2±1.2g)。结论:抗阻训练能使骨骼肌的细胞核增加,进而使骨骼肌的力量和体积增大。

Impact of resistant training on amount of nascent nuclei of aging rats' skeletal muscles

Objective:To observe the impact of 10-week resistant training on the amount of nascent nuclei of skeletal muscles in aging rats,and strengthening of it’s synthesis ability.Method:Forty SD rats(17 month old) were selected randomly divided into a quiet group as well as large,medium and low loading training groups,with 10 rats in each group.The nascent myonucle:were marked with 5-bromodeoxyuridine(Brdu).The amount of nascent myonuclei were compared with immunohistochemical method.Result:The amounts of nascent myonuclei in large loading training group(7.5±4.2) and moderate loading training group(6.7±3.6)were significantly higher than that in control group(2.1±1.3)and low loading training group(5.1±2.6).The average quantity of myonuclei in muscle fiber in large loading training group(3.2±0.6) and moderate loading training group(3.1±0.5) were significantly higher than that in control group(2.4±0.5)and low loading training group(2.8±0.6).The weight of skeletal muscle in moderate loading training group(10.6±1.3g) and large loading training group(10.1±1.4g) were significantly higher than that in control group(8.2±1.2g)Conclusion:Resistant training could increase the amount of skeletal muscle nuclei,so as to enhance the strength of skeletal muscle and the volume of skeletal muscle.