RP-HPLC测定祛风止痛片中原儿茶酸和原儿茶醛的含量

目的：建立同时测定祛风止痛片中原儿茶酸和原儿茶醛含量的方法。方法：采用RP-HPLC法,色谱条件为LunaC18色谱柱（4.6mm×250mm,5μm）,流动相为乙腈-0.2%磷酸溶液（16：84）,流速：0.8mL·min^-1,柱温：30℃,检测波长：280nm。结果：原儿茶酸和原儿茶醛分别在0.62～12.40μg·mL^-1（r=0.9997）和0.14～2.80μg·mL^-1（r=0.9997）浓度范围内均有良好的线性关系,平均加样回收率分别为96.9%、97.9%;RSD均小于2.0%。结论：本方法准确,重复性好,可用于该制剂的质量控制。

Determination of Protocatechuic Acid and Protocatechualdehyde in Qufeng Zhitong Tablets by RP-HPLC

Objective：To establish a method for the determination of protocatechuic acid and protocatechualdehyde in Qufeng Zhitong Tablets.Method：The RP-HPLC system consisting the Luna C18（4.6mm×250mm,5μm）column,the mobile phase consisted of Acetonitrile-0.2%H3PO4（16：84）,the flow rate was 0.8mL·min^-1,the column temperature was 30℃,The UV detector was set at 280nm.Results：The linear response range was 0.62～12.40μg·mL^-1（r=0.9997）and 0.14～2.80μg·mL^-1（r=0.9997）,respectively.The average recovery of protocatechuic acid and protocatechualdehyde were 96.9% and 97.9%,respectively,RSD2.0%.Conclusions：The assay demonstrated that the method had adequate and selectivily to measure the concentrations of accurate,repeatable,it can be applied in quantitative determination of protocatechuic acid and protocatechualdehyde in Qufeng Zhitong Tablets.