嗅三针对血管性痴呆大鼠学习记忆功能及海马ChAT AchE活性的影响

目的：探讨嗅三针对血管性痴呆大鼠学习记忆功能及海马ChAT、AchE活性的影响作用。方法：成年SD雄性大鼠40只,体重（300±20）g。随机分为正常对照组、VD模型组、VD嗅球损毁模型组、嗅三针组,每组10只。制作VD大鼠模型和VD并损毁嗅球大鼠模型,通过嗅三针电刺激方法,测试大鼠水迷宫学习记忆能力及海马胆碱乙酰化酶（ChAT）、乙酰胆碱酯酶（AchE）活性。结果：水迷宫定位航行试验显示,平均逃避潜伏期和平均游泳路程比较,VD模型组明显长于正常对照组（P〈0.01）;嗅三针组明显短于VD模型组和VD嗅球损毁模型组（P〈0.01）;VD嗅球损毁模型组与VD模型组之差异无显著性意义（P〉0.05）。海马ChAT、AchE活性比较,VD模型组低于正常对照组（P〈0.05）;嗅三针组高于VD模型组和VD嗅球损毁模型组（P〈0.05）;VD模型组与VD嗅球损毁模型组之差异无显著性意义（P〉0.05）。结论：嗅三针能够显著增强血管性痴呆大鼠学习记忆功能,并且能提高海马ChAT和AchE活性,其治疗效应依赖于嗅觉传导通路的完整性。

Effect of XIU Three Needles on Learning-Memory Ability and ChAT AchE Activity of Hippocampus in Vascular Dementia Rats

Objective：To explore the effect of XIU Three Needles on learning-memory ability and chorine acetyl transferees（ChAT） ,acetyl cholinesterase（AchE） activity of hippocampus in vascular dementia（VD） rats.Methods：The 40 Sprague Dawley rats were randomly divided into control,VD model,VD plus removed olfactory bulb model and XIU Three Needles groups.VD models were established by 4-vessel occlusion.Morris maze tests were conducted for valuating the learning and memory abilities.The activity of ChAT and AChE of hippocampus was detected by colorimetric method.Results：In comparison with control group,the average escape latency and swim distance prolonged significantly in VD model group（P〈0.01） .In comparison with VD model group,the average escape latency and swim distance shortened remarkably in XIU Three Needles group（P〈0.01） .No significant differences were found between VD model and VD plus removed olfactory bulb model groups in the average escape latency and swim distance（P〉0.05） .The ChAT and AchE activity of hippocampus in VD model group were lower than those of control group（P〈0.05） ,while the ChAT and AchE activity of hippocampus in VD model group were lower than those of XIU Three Needles group（P〈0.05） .No significant differences were found between VD model and VD plus removed olfactory bulb model groups in the ChAT and AchE activity of hippocampus（P〈0.05） . Conclusion：The effect of XIU Three Needles dramatically increased the learning-memory abilities of VD rats,and could increase the ChAT and AchE activity of hippocampus in VD rats at the same time.Its therapeutical effects depended on the entirety of olfactory pathway.