| 胃癌中性糖脂的分离及纯化 |
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| 引用本文： | 张积仁,张德胜.胃癌中性糖脂的分离及纯化[J].第四军医大学学报,1989,10(6):370-371. |
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| 作者姓名： | 张积仁 张德胜 |
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| 作者单位： | 西京医院消化病研究室
(张积仁，张学庸，陈希陶)，上海医科大学生物化学教研室
(张德胜)，西京医院消化病研究室(顾天爵) |
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| 摘 要： | 作者应用甲醇氯仿抽提胃癌总脂,经sephadex-A25离子交换层析,florisil层析的方法从28g胃癌组织中分离纯化出中性糖脂(?)1.1mg,经高效薄层色谱分析,与标准品对照证实,条带清楚,纯度可靠。为胃癌中性糖脂抗原的研究打下了基础。
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| 关 键 词： | 胃肿瘤 糖脂类 薄层色谱法 |
Isolation and Purification of Neutral Glycolipids from Gastric Cancer Tissue |
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| Zhang Jiren,Zhang Desheng Zhang Xueyong,Chen Xitao and Gu Tianjue Laboratory of Gastroenterology,Xijing Hospital.Isolation and Purification of Neutral Glycolipids from Gastric Cancer Tissue[J].Journal of the Fourth Military Medical University,1989,10(6):370-371. |
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| Authors: | Zhang Jiren Zhang Desheng Zhang Xueyong Chen Xitao and Gu Tianjue Laboratory of Gastroenterology Xijing Hospital |
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| Institution: | Zhang Jiren,Zhang Desheng Zhang Xueyong,Chen Xitao and Gu Tianjue Laboratory of Gastroenterology,Xijing Hospital Department of Biochemistry,Shanghai Medical University |
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| Abstract: | Neutral glycolipids were purified from human gastric cancer tissue. The lipids were extracted from cancer tissue with chloroform/methanol. Total lipids were loaded on a DEAE-sephadex-A25 column. The neutral glycolipids were eluted with chloroform/methanol/water (60:30:8, v/v/v), and further purified by florisil column chromatography. By comparing with standard neutral glycolipids of red blood cell membrane, the neutral glycolipids purified from gastric cancer tissue were analysed on high performance thin layer chromatography, and confirmed that it has been purified. This method may be used to study the neutral glycolipids of gastric cancer. |
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| Keywords: | glycolipids stomach neoplasms chromatography thin layer |
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