预先电针对实验性类痛经模型大鼠血浆血栓素B2及6-酮-前列腺素F_(1α)的影响

目的:观察电针预先介入对实验性类痛经大鼠血浆血栓素2(TXB2)及6-酮-前列腺素F1α(6-keto-PGF1α)的影响,初步探讨经穴调控胞宫疼痛的特异性。方法:SD大鼠随机分为盐水组、模型组、三阴交组、血海组、悬钟组、非穴组。采用苯甲酸雌二醇和缩宫素制备类痛经模型。电针组给予电针20min,每日1次,连续3d,盐水组和模型组不予电针处理。末次电针后观察大鼠扭体反应,并检测血浆TXB2、6-keto-PGF1α的含量。结果:与盐水组比较,模型组扭体潜伏期显著缩短(P0.01),扭体评分显著增高(P0.05),血浆TXB2含量、TXB2/6-keto-PGF1α比值均显著升高(P0.05);与模型组比较,三阴交组TXB2/6-keto-PGF1α比值显著降低(P0.05);各电针组之间比较,三阴交组的扭体评分较血海组、非穴组显著降低(P0.05)。结论:预先电针三阴交穴可能通过调节血浆TXB2/6-keto-PGF1α的失衡,改善血管内环境,缓解平滑肌的痉挛状态,进而缓解胞宫疼痛,血海穴、悬钟穴、非穴的调节作用不明显,推测三阴交穴具有调控胞宫疼痛的相对特异性。

Effect of pre-electroacupuncture at different acupoints on plasma TXB2、6-keto-PGF1α in dysmenorrhea rats

Objective: To observe the effect of pre-electroacupuncture(EA) on plasma TXB2,6-keto-PGF1α in dysmenorrhea rats in order to discuss the specificity of acupoints in relieving uterus pain preliminarily.Methods: Sprague-Dawley(SD) rats were randomly divided into saline group,model group,EA Sanyinjiao(SP6) group,EA Xuehai(SP10) group,EA Xuanzhong(GB39) group,EA non-acupoint(NA) group.Estradiol benzoate and oxytocin were chosen to make the model of dysmenorrhea.The rats of treatment groups were given EA for 20 minutes after bounded,while,the rats of saline group and model group were only bounded for 20 minutes,once a day,for 3 days.The latency period and score of writhing were recorded for 20 minutes after the last EA.The content of Plasma TXB2、6-keto-PGF1α were detected with radioimmunoassay.Results: Compared with saline group,the latency period of writhing in model group was significantly shortened(P<0.01),and the writhing score was significantly increased(P<0.05).The content of plasma TXB2,and the ratio of TXB2/6-keto-PGF1α in model group were significantly increased(P<0.05).In comparison with model group,the ratio of TXB2/6-keto-PGF1α in SP6 group was significantly reduced(P<0.05).The writhing score in SP6 group was significantly increased compared with SP10 group and NA group.Conclusion: Pre-EA SP6 can balance plasma TXB2/6-keto-PGF1α to change the environment of blood vessel,relieve spasm of blood vessels to ease the pain,there weren’t obvious regulation with SP10,GB39 and NA.SP6 had better effect for regulating vein substance,SP6 had the specificity among acupoints in relieving uterus pain.