持续弹性外牵引对雌性小型猪乳头碱性成纤维细胞生长因子和β1转化生长因子表达的影响

目的观察持续弹性外牵引过程中雌性小型猪乳头及其支撑组织中碱性成纤维细胞生长因子（bFGF）和β1转化生长因子（TGF-β1）表达的变化，探讨持续弹性外牵引矫治乳头内陷的机制。方法选用3只雌性小型猪，每只各12个乳头；随机选取4个乳头作为空白对照组，其余乳头安置自制弹性可调式牵拉矫治器进行持续外牵引。于牵引后第2、4、8、12周分别各切取1个空白对照组乳头和2个被牵引乳头，行免疫组织化学染色，观察不同时期bFGF和TCF-β1的表达情况。结果牵引后各时期bFGF及TGF-β1的表达均较对照组明显增加，差异有统计学意义（P〈0．01）。bFGF的阳性表达在牵引4周时达到最高水平，与12周时相比差异有统计学意义（P〈0．05）。TOF-β1的阳性表达在牵引2周时达到最高水平，同8周、12周牵引组相比差异有统计学意义（P〈0．05）。bFGF和TGF-β1表达呈显著正相关。结论持续弹性外牵引可刺激小型猪乳头组织中bFGF和TGF-β1的合成和分泌增加。

Effects of continuous elastic outside distraction on expression of basic fibroblast growth factor and trans- forming growth factor-beta 1 in nipple of female minipig model

Objective To observe the effect of continuous elastic outside distraction on the ex- pression of basic fibroblast growth factor （bFGF） and transforming growth factor-beta 1 （TGF-β1） in the female porcine nipples, and to further investigate the mechanism of continuous elastic outside dis- traction stimulation on the proliferation of supporting tissue in nipples. Methods Three female minip- igs were employed, each of them had 12 nipples. Four nipples of each minipig were used as control group, while the other eight nipples were continuously distracted with prefabricated instruments. One nipple without distraction and two distracted nipples of each minipig were excised at 2, 4, 8, and 12 weeks after operation under general anesthesia respectively. The excised nipples were prepared for im- munohistochemical staining to observe the expression of bFGF and TGF-β1. Image analysis software was employed for quantitative analysis. Results bFGF and TGF-β1 expression of control groups was weaker than those of the distracted groups at any time （P〈0.01）. The expression of bFGF increased gradually after tracted and reached the peak at 4 weeks. There was significant difference （P〈0.05） in the expression of bFGF between 4 weeks and 12 weeks groups. The expression of TGF-β1 was in- creased to the peak after 2 weeks. The expression of TGFβ1 between 8 weeks and 12 weeks groups and 2 weeks group had statistical significance （P〈0.05）. The expression of bFGF and TGF-β1 had a positive correlation during the traction. Conclusions Continuous elastic outside distraction can pro- mote female minipig nipple to synthesis and secretion of b-FGF and TGF-β1.