两株双价痢疾工程菌苗不同粘膜免疫途径的免疫原性

目的探讨免疫途径、接种剂量及侵袭蛋白表达对两株双价痢疾工程菌苗免疫效果的影响。方法小鼠分为滴鼻、灌胃和对照组 ,分别以不同剂量免疫 3次 ,间隔 2周 ,3次免疫后 7d收集血清、小肠、鼻咽、肺、阴道冲洗液 ,EL ISA法检测其中特异性福氏、宋内氏 L PS- Ig A和 Ig G。结果 4× 10 7CFU菌苗经鼻途径免疫即可诱导多个粘膜部位以及血清双价特异性抗体显著增高 ;菌苗剂量增加 2 0、2 0 0倍时经灌胃免疫诱发较为局限的粘膜特异性抗体增高。结论鼻粘膜免疫不仅诱导多个粘膜部位 (特别是生殖道 )以及系统免疫的抗体反应 ,是一个安全有效的免疫途径。

Immunogenicity of two bivalent Shigella vaccines after intranasal or intragastric administration in mice

Objective To observe the effect of two different mucosaladministration routes to the immunogenicity of two bivalent Shigella vaccines. Methods BALB/c mice were divided into 3 groups, 20 mice per group. Mice were immunized respectively with Ipa+ or Ipa－ vaccines 3 times with an interval of 2 weeks by intranasal or intragastric route.The serum, nasopharynx wash, lung wash, small intestinal wash and vaginal wash were collected. The amounts of IgA and IgG against flexineri and sonnei LPS were measured by ELISA. Results The levels of specific antibody in nasopharynx wash, lung wash, small intestinal wash,vaginal wash and serum were significantly increased after intranasal immunization with 4×107 CFU of two bivalent Shigella vaccines.When the dosage increased 20 or 200 times,the levels of specific antibody in nasopharynx wash,lung wash,small intestinal wash,vaginl wash and serum were significantly increased after intragastric immunization．Conclusions Two bivalent Shigella vaccines can induce system and several local mucosal immunity reaction by nasal mucosa administration routes in low dosage compared with intragastric route(about 1/20).