肾小球肾炎患者外周血淋巴细胞Bcl-2和Fas的表达

评价外周血淋巴细胞凋亡在肾小球肾炎（GN）发病中的作用。方法用免疫组化、荧光显微镜及电子显微镜对GN进行病理分型，采用流式细胞术测定不同病理类型GN患者外周血单个核细胞（PBMC）Bcl－2、Fas表达水平。结果各型GNPBMCBcL－2表达水平均高于对照组（P＜0．01），Fas均低于对照组（P＜0．01）；增殖型GN与非增殖型GN及增殖型GN中IgAN、MPGN、HSPN新月体组与非月体组间，Bcl－2、Fas表达水平存在显著差异，前者Bcl-2高于后者（P＜0．01），Fas低于后者（P＜0．01）。结论Bcl－2过量表达，Fas系统功能不良，活化PBMC增多，导致肾小球肾炎。Bcl－2、Fas表达水平与GN病理类型及临床预后有一定联系。

Expression of Bcl-2 protein and fas antigen in peripherial blood lymphocytes for children with glomerulonephritis

To study the effect of peripherial blood lymphocytesapoptosis in the development of glomerulonephritis (GN).Results: We classified glomerulonephritis using electric microscope, fluorescence microscope and immunohistochemistry, and investigated expression of both Bcl-2 protein and Fas antigen in peripherial blood mononuclear cells (PBMC)in patients with differentpathologic types of GN using flow cytometry.Results: In comparison with those in controls, expressive levelof Bcl-2 protein in PBMC was significantly higher in every type ofGN and Fas antigen was significantly lower (P<0. 01). There werestrikingly differences between proliferative GN (PGN) and non-proliferative GN (NPGN) as well as between crescent group and noncrescent group in PGN including IgA nephropathy (IgAN), membranous proliferative GN (MPGN) and Honch-Shonlein purpura nephritis (HSPN). Expressive levels of BcL-2 in PBMC in PGN and crescent group of PGN were remarkably higher than those in NPGN andnon-crescent group of PGN (P<0. 01). In contrast with these, Fasantigen was significantly lower (P<0.01).Conclusion GN was caused by overexpression of Bcl-2 protein,loss-of-fuction of Fas system and increase of activated PBMC. Expressive levels of both Bcl-2 protein and Fas antigen were associated with pathologic types ofGN and clinical result.