bcr／ab1融合基因在慢性粒细胞白血病中的表达及临床意义

目的探讨bcr／ab1融合基因的表达在慢性粒细胞白血病（CML）中的诊断、疗效及预后观察中的意义。方法采用荧光定量逆转录多聚酶链反应（FQ-PCR）技术，对8例复治CML患者和作为对照组的2例急性非淋巴细胞性白血病患者的骨髓或外周血进行bcr／ab1融合基因检测；同时比较患者的血象、骨髓象等相关实验室指标。结果CML中7例慢性期（CP）和1例急变期（BC）患者，均不同程度地表达了bcr／ab1融合基因，而对照组该融合基因的表达为阴性。结论bcr／ab1基因是CML发病的分子基础，定量检测bcr／ab1融合基因对于CML的诊断、临床分型、疗效观察及预后判断有重要意义。

Expression of bcr/ab1 fusion gene in chronic myeloid leukemia and its clinical significance

Objective To study the significance of bcr/abl fusion gene expression in the diagnosis,treatment and prognosis in chronic myeloid leukemia(CML).Methodsbcr/abl fusion gene of bone marrow or peripheral blood samples from 8 cases with CML and 2 cases with ANLL were amlified by FQ-PCR.ResultsAll of the cases expression bcr/abl fusion gene out of 7 cases of chronic phase(CP) and 1 case of blast crisis(BC) with CML.2 cases with ANLL were bcr/abl fusion gene negative.ConclusionThere has important significance in the diagnosis,clinical typing,treatment and prognosis in CML using bcr/abl gene quantitative detecting technique.